Application
Fig1: Western blot analysis of p150 CAF1 on K562 (1) and A431 (2) cell lysate using anti-p150 CAF1 antibody at 1/1,000 dilution.Application
Fig2: ICC staining p150 CAF1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining p150 CAF1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining p150 CAF1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p150 CAF1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-p150 CAF1 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Daudi cells with p150 CAF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | p150 CAF1 [JG38-98] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAF 1 antibody CAF 1 subunit A antibody CAF antibody CAF I 150 kDa subunit antibody CAF I p150 antibody CAF-1 subunit A antibody CAF-I 150 kDa subunit antibody CAF-I p150 antibody CAF1 antibody CAF1A_HUMAN antibody CAF1B antibody CAF1P150 antibody CHAF1A antibody Chromatin assembly factor 1 subunit A antibody Chromatin Assembly Factor 1 Subunit A p150 antibody Chromatin assembly factor I (150 kDa) antibody Chromatin assembly factor I 150 kDa antibody Chromatin assembly factor I p150 subunit antibody DCAF1 antibody hp150 antibody MGC71229 antibody P150 antibody |
| Product description | Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated Histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated. |
| Immunogen | Recombinant protein within human p150 CAF1 aa 100-300. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIPIHCFC |
| WB:1:500-1:2,000 ICC/IF:1:50-1:200 IHC:1:50-1:200 IP:1:10-1:50 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CAF 1 antibody CAF 1 subunit A antibody CAF antibody CAF I 150 kDa subunit antibody CAF I p150 antibody CAF-1 subunit A antibody CAF-I 150 kDa subunit antibody CAF-I p150 antibody CAF1 antibody CAF1A_HUMAN antibody CAF1B antibody CAF1P150 antibody CHAF1A antibody Chromatin assembly factor 1 subunit A antibody Chromatin Assembly Factor 1 Subunit A p150 antibody Chromatin assembly factor I (150 kDa) antibody Chromatin assembly factor I 150 kDa antibody Chromatin assembly factor I p150 subunit antibody DCAF1 antibody hp150 antibody MGC71229 antibody P150 antibody |
|---|---|
| Molecular Weight(MW) | 107 kDa (Predicted band size) |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q13111 |
|---|
Application
Fig1: Western blot analysis of p150 CAF1 on K562 (1) and A431 (2) cell lysate using anti-p150 CAF1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining p150 CAF1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining p150 CAF1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining p150 CAF1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p150 CAF1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-p150 CAF1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Daudi cells with p150 CAF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | K562, A431, 293T, LOVO, human tonsil tissue, human colon tissue, Daudi. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC/IF:1:50-1:200 IHC:1:50-1:200 IP:1:10-1:50 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
