Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Siha(Human) Cell Lysate at 30 ug Molt-4(Human) Cell Lysate at 30 ug Primary: Anti- BRD8 (ER1904-32) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 135 kD Observed band size: 130 kD| Product Name | BRD8 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRD8 Bromodomain containing 8 p120 Skeletal muscle abundant protein 2 Skeletal Muscle Abundant Protein SMAP SMAP2 Thyroid hormone receptor coactivating protein 120kDa Thyroid Hormone Receptor Coactivating Protein BRD8_HUMAN. |
| Product description | BRD8 may act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. It interacts with MYC and adenovirus E1A protein. |
| Immunogen | KLH conjugated synthetic peptide derived from human BRD8 331-430/1235 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | BRD8 Bromodomain containing 8 p120 Skeletal muscle abundant protein 2 Skeletal Muscle Abundant Protein SMAP SMAP2 Thyroid hormone receptor coactivating protein 120kDa Thyroid Hormone Receptor Coactivating Protein BRD8_HUMAN. |
|---|---|
| Molecular Weight(MW) | 135 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q9H0E9 |
|---|
Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Siha(Human) Cell Lysate at 30 ug Molt-4(Human) Cell Lysate at 30 ug Primary: Anti- BRD8 (ER1904-32) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 135 kD Observed band size: 130 kD| Positive Control | Expressed in adipose tissue, brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
