Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Spinal cord (Mouse) Lysate at 40 ug Primary: Anti-BRN3A (ER1904-34) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 33 kDApplication
Fig2: U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BRN3A Antibody(ER1904-34)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).| Product Name | BRN3A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Brain specific homeobox/POU domain protein 3A Brn 3.0 BRN 3A Brn3 Homeobox/POU domain protein RDC 1 Homeobox/POU domain protein RDC1 Oct T1 OctT1 POU Domain Class 4 Transcription Factor 1 POU4F1 RDC 1 RDC1. |
| Product description | Multifunctional transcription factor with different regions mediating its different effects. Acts by binding (via its C-terminal domain) to sequences related to the consensus octamer motif 5'-ATGCAAAT-3' in the regulatory regions of its target genes. Regulates the expression of specific genes involved in differentiation and survival within a subset of neuronal lineages. It has been shown that activation of some of these genes requires its N-terminal domain, maybe through a neuronal-specific cofactor. Ativates BCL2 expression and protects neuronal cells from apoptosis (via the N-terminal domain). Induces neuronal process outgrowth and the coordinate expression of genes encoding synaptic proteins. Exerts its major developmental effects in somatosensory neurons and in brainstem nuclei involved in motor control. Stimulates the binding affinity of the nuclear estrogene receptor ESR1 to DNA estrogen response element (ERE), and hence modulates ESR1-induced transcriptional activity. May positively regulate POU4F2 and POU4F3. Regulates dorsal root ganglion sensory neuron specification and axonal projection into the spinal cord. Plays a role in TNFSF11-mediated terminal osteoclast differentiation. Negatively regulates its own expression interacting directly with a highly conserved autoregulatory domain surrounding the transcription initiation site. |
| Immunogen | KLH conjugated synthetic peptide derived from human BRN3A 325-419/419 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1ug/test: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Brain specific homeobox/POU domain protein 3A Brn 3.0 BRN 3A Brn3 Homeobox/POU domain protein RDC 1 Homeobox/POU domain protein RDC1 Oct T1 OctT1 POU Domain Class 4 Transcription Factor 1 POU4F1 RDC 1 RDC1. |
|---|---|
| Molecular Weight(MW) | 43 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q01851 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Spinal cord (Mouse) Lysate at 40 ug Primary: Anti-BRN3A (ER1904-34) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 33 kD
Application
Fig2: U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BRN3A Antibody(ER1904-34)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).| Positive Control | Brain. Seems to be specific to the retina. Present in the developing brain, spinal cord and eye. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1ug/test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
