Application
Fig1: Blank control: U937(blue). Primary Antibody: Rabbit Anti-BST1 antibody(ER1904-36), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min).Primary antibody (ER1904-36, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.Application
Fig2: Sample: Raw264.7(Mouse) Cell Lysate at 30 ug Primary: Anti-BST1 (ER1904-36) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD| Product Name | BST1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cyclic ADP ribose hydrolase 2 ADP ribosyl cyclase 2 Bone marrow stromal antigen 1 Bone marrow stromal cell antigen 1 BST 1 BST1 BST-1 cADPr hydrolase 2 CD157 CD157 antigen NAD(+) nucleosidase BST1_HUMAN. |
| Product description | Synthesizes cyclic ADP-ribose, a second messenger that elicits calcium release from intracellular stores. May be involved in pre-B-cell growth. |
| Immunogen | KLH conjugated synthetic peptide derived from human BST1/CD157 51-150/318 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Cyclic ADP ribose hydrolase 2 ADP ribosyl cyclase 2 Bone marrow stromal antigen 1 Bone marrow stromal cell antigen 1 BST 1 BST1 BST-1 cADPr hydrolase 2 CD157 CD157 antigen NAD(+) nucleosidase BST1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 33 KDa |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. |
| SwissProt ID | Q10588 |
|---|
Application
Fig1: Blank control: U937(blue). Primary Antibody: Rabbit Anti-BST1 antibody(ER1904-36), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min).Primary antibody (ER1904-36, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: Raw264.7(Mouse) Cell Lysate at 30 ug Primary: Anti-BST1 (ER1904-36) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD| Positive Control | Widely expressed. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
