Application
Fig1: Sample:
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti-BSX (ER1904-37) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD
Application
Fig2: Sample:
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti-BSX (ER1904-37) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD
Application
Fig3: Blank control: RSC96(blue).
Primary Antibody:Rabbit Anti-BSX antibody(ER1904-37), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody(blue):Rabbit IgG(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.