Application
Fig1: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-CBFb antibody. Counter stained with hematoxylin.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CBFb antibody. Counter stained with hematoxylin.Application
Fig3: Flow cytometric analysis of SiHa cells with CBFb antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | CBFb [JG39-35] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CBF b antibody CBF beta antibody CBF-beta antibody CBFB antibody CBFbeta antibody Core binding factor beta subunit antibody core binding factor subunit beta antibody Core-binding factor subunit beta antibody PEA 2 antibody PEA2 antibody PEA2 beta antibody PEA2-beta antibody PEA2beta antibody PEBB_HUMAN antibody PEBP 2B antibody PEBP2 beta antibody PEBP2-beta antibody PEBP2B antibody PEBP2beta antibody Polyomavirus enhancer binding protein 2 beta subunit antibody Polyomavirus enhancer-binding protein 2 beta subunit antibody SL3 3 enhancer factor 1 beta subunit antibody SL3 3 enhancer factor 1 subunit beta antibody SL3-3 enhancer factor 1 subunit beta antibody SL3/AKV core binding factor beta subunit antibody SL3/AKV core-binding factor beta subunit antibody |
| Product description | The transcription factor Polyomavirus enhancer binding protein 2 (PEBP2), also designated Osf2 (Osteoblast-specific transcription factor), CBFA1 (Core Binding Factor) and AML3 (Acute myeloid leukemia), is composed of two subunits, a and b, which are essential for the regulation of hematopoiesis and osteogenesis. The PEBP2a subunits, PEBP2aA, PEBP2aB and PEBP2aC, are encoded by three RUNX genes, all of which contain a 128-amino acid region homologous to the highly conserved Drosophila segmentation gene, Runt. This region is involved in DNA binding and heterodimerization with the regulatory b subunit, which facilitates DNA binding of the a subunit. Both subunits are required for in vivo function; the disruption of either gene results in a lack of definitive hematopoiesis followed by embryo death in utero due to hemorrhage in the central nervous system. The gene encoding PEBP2b is the target of chromosomal inversion 16 (p13;q22) with the smooth muscle myosin heavy chain, producing a chimeric gene, PEBP2b/CBFB-SMMHC, that is associated with human acute myeloid leukemia. |
| Immunogen | Recombinant protein within human CBFb aa 1-200. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCFC |
| IHC:1:50-1:200 FC:1:50-1:100 WB:1:500 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | CBF b antibody CBF beta antibody CBF-beta antibody CBFB antibody CBFbeta antibody Core binding factor beta subunit antibody core binding factor subunit beta antibody Core-binding factor subunit beta antibody PEA 2 antibody PEA2 antibody PEA2 beta antibody PEA2-beta antibody PEA2beta antibody PEBB_HUMAN antibody PEBP 2B antibody PEBP2 beta antibody PEBP2-beta antibody PEBP2B antibody PEBP2beta antibody Polyomavirus enhancer binding protein 2 beta subunit antibody Polyomavirus enhancer-binding protein 2 beta subunit antibody SL3 3 enhancer factor 1 beta subunit antibody SL3 3 enhancer factor 1 subunit beta antibody SL3-3 enhancer factor 1 subunit beta antibody SL3/AKV core binding factor beta subunit antibody SL3/AKV core-binding factor beta subunit antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q13951 |
|---|
Application
Fig1: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-CBFb antibody. Counter stained with hematoxylin.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CBFb antibody. Counter stained with hematoxylin.
Application
Fig3: Flow cytometric analysis of SiHa cells with CBFb antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Rat kidney tissue, human kidney tissue, SiHa. |
|---|---|
| Application Notes | IHC:1:50-1:200 FC:1:50-1:100 WB:1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
