Application
Fig1: Western blot analysis of SUN2 on rat kidney tissue lysate using anti-SUN2 antibody at 1/2,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-SUN2 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SUN2 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-SUN2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-SUN2 antibody. Counter stained with hematoxylin.Application
Fig6: ICC staining SUN2 (green) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-SUN2 antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of SK-Br-3 cells with SUN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | SUN2 [JG39-52] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FRIGG antibody KIAA0668 antibody nuclear envelope protein antibody Protein unc-84 homolog B antibody Rab5 interacting protein antibody RAB5IP antibody Sad1 and UNC84 domain containing 2 antibody Sad1 unc-84 domain protein 2 antibody Sad1 unc84 domain protein antibody Sad1/unc-84 protein-like 2 antibody Sad1/unc84 protein-like antibody SUN domain-containing protein 2 antibody UNC 84B antibody unc84 homolog B (C. elegans) antibody unc84 homolog B antibody unc84, C. elegans, homolog of, B antibody UNC84B antibody |
| Product description | SUN2 (sad1/unc-84 protein-like 2), also known as UNC84B (unc-84 homolog B), FRIGG, KIAA0668 or RAB5IP, is a 717 amino acid single-pass membrane protein that contains one SUN domain and localizes to the membrane of both the nucleus and the endosome. Widely expressed in a variety of tissues, including lung, muscle and heart, SUN2 interacts with Rab 5A and may play a role in homotypic endosome fusion. The gene encoding SUN2 maps to human chromosome 22, which houses over 500 genes and is the second smallest human chromosome. Mutations in several of the genes that map to chromosome 22 are involved in the development of Phelan-McDermid syndrome, Neurofibromatosis type 2, autism and schizophrenia. Additionally, translocations between chromosomes 9 and 22 may lead to the formation of the Philadelphia Chromosome and the subsequent production of the novel fusion protein BCR-Abl, a potent cell proliferation activator found in several types of leukemias. |
| Immunogen | Recombinant protein within human SUN2 aa 500-700. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCFCICCIF |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 ICC/IF:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | FRIGG antibody
KIAA0668 antibody
nuclear envelope protein antibody
Protein unc-84 homolog B antibody
Rab5 interacting protein antibody
RAB5IP antibody
Sad1 and UNC84 domain containing 2 antibody
Sad1 unc-84 domain protein 2 antibody
Sad1 unc84 domain protein antibody
Sad1/unc-84 protein-like 2 antibody
Sad1/unc84 protein-like antibody
SUN domain-containing protein 2 antibody
UNC 84B antibody
unc84 homolog B (C. elegans) antibody
unc84 homolog B antibody
unc84 C. elegans homolog of B antibody UNC84B antibody |
|---|---|
| Molecular Weight(MW) | 80 kDa |
| Cellular Localization | Endosome. Nucleus inner membrane. |
Application
Fig1: Western blot analysis of SUN2 on rat kidney tissue lysate using anti-SUN2 antibody at 1/2,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
Application
Fig6: ICC staining SUN2 (green) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of SK-Br-3 cells with SUN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Rat kidney tissue lysate, rat epididymis tissue, human tonsil tissue, human colon tissue, human prostate cancer tissue, mouse liver tissue, SK-Br-3. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 ICC/IF:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
