Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (C17orf75) Polyclonal Antibody, Unconjugated (ER1904-54) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig2: Sample: U2os Cell (Human) Lysate at 30 ug Primary: Anti- C17orf75 (ER1904-54)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45kD Observed band size: 45kD| Product Name | C17orf75 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Njmu R1 Protein kinase Njmu R1 Protein Njmu R1 NJMU_HUMAN. |
| Immunogen | KLH conjugated synthetic peptide derived from human C17orf75 201-300/396 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Njmu R1 Protein kinase Njmu R1 Protein Njmu R1 NJMU_HUMAN. |
|---|---|
| Molecular Weight(MW) | 45 KDa |
| SwissProt ID | Q9HAS0 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (C17orf75) Polyclonal Antibody, Unconjugated (ER1904-54) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig2: Sample: U2os Cell (Human) Lysate at 30 ug Primary: Anti- C17orf75 (ER1904-54)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45kD Observed band size: 45kD| Positive Control | The molecular function and cellular component of this protein are unknown. It may have a role in spermatogenesis, is highly expressed in testis and is also expressed in fetal testis. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
