Application
Fig1: Western blot analysis of Nectin 2 on K562 (1), MCF-7 (2) and SK-OV-3 (3) cells lysates using anti-Nectin 2 antibody at 1/500 dilution.Application
Fig2: ICC staining Nectin 2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Nectin 2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Nectin 2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Nectin 2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nectin 2 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of LOVO cells with Nectin 2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Nectin 2 [JG39-64] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 112 antibody CD112 antibody CD112 antigen antibody Herpes virus entry mediator B antibody Herpes virus entry protein B antibody Herpesvirus entry mediator B antibody Herpesvirus entry protein B antibody Hve B antibody HveB antibody Nectin-2 antibody Nectin2 antibody Poliovirus receptor like 2 antibody Poliovirus receptor related 2 (herpesvirus entry mediator B) antibody Poliovirus receptor related 2 antibody Poliovirus receptor related protein 2 antibody Poliovirus receptor-related protein 2 antibody PRR 2 antibody PRR2 antibody PVRL 2 antibody PVRL2 antibody PVRL2_HUMAN antibody PVRR 2 antibody PVRR2 antibody |
| Product description | Nectin is a Ca2+-independent homophilic cell adhesion molecule that belongs to the immunoglobulin superfamily. Human nectin is identical to the poliovirus receptor-related protein (PRR) and has been identified as the α-herpesvirus entry mediator. Nectin constitutes a family consisting of at least Nectin 1, 2 and 3; each member has two or three splicing variants. Nectin 2, also designated PRR2/HveB, is ubquitously expressed, with the highest levels of expression in some human neuronal cell lines, fibroblastic cells, keratinocytes and primary activated T lymphocytes. Nectin 2 has two splicing variants, Nectin 2α (short form) and 2δ (long form). Both Nectin 2α and 2δ have a C-terminal conserved motif (E/A-X-Y-V). This motif interacts with the PDZ domain of the F-Actin-binding protein afadin, through which it is linked to the Actin cytoskeleton. The extracellular regions of the splicing variants are identical, but their transmembrane regions and cytoplasmic regions are unique. Nectin 2 mediates the entry of three mutant herpes simplex virus type 1 (HSV-1) strains that do not use HveA as co-receptor, but not wildtype HSV-1 strains. Nectin 2 also mediates the entry of HSV-2 and pseudorabies virus, but not bovine herpes virus type 1. Nectin 2δ is tyrosine phosphorylated in response to cell-cell adhesion. |
| Immunogen | Recombinant protein within human Nectin 2 aa 50-250. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFC |
| WB:1:500-1:2000 ICC:1:50 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CD 112 antibody CD112 antibody CD112 antigen antibody Herpes virus entry mediator B antibody Herpes virus entry protein B antibody Herpesvirus entry mediator B antibody Herpesvirus entry protein B antibody Hve B antibody HveB antibody Nectin-2 antibody Nectin2 antibody Poliovirus receptor like 2 antibody Poliovirus receptor related 2 (herpesvirus entry mediator B) antibody Poliovirus receptor related 2 antibody Poliovirus receptor related protein 2 antibody Poliovirus receptor-related protein 2 antibody PRR 2 antibody PRR2 antibody PVRL 2 antibody PVRL2 antibody PVRL2_HUMAN antibody PVRR 2 antibody PVRR2 antibody |
|---|---|
| Molecular Weight(MW) | 58 kDa |
| Cellular Localization | Cell membrane. Membrane. |
Application
Fig1: Western blot analysis of Nectin 2 on K562 (1), MCF-7 (2) and SK-OV-3 (3) cells lysates using anti-Nectin 2 antibody at 1/500 dilution.
Application
Fig2: ICC staining Nectin 2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Nectin 2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Nectin 2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Nectin 2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nectin 2 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of LOVO cells with Nectin 2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | K562, MCF-7, SK-OV-3, LOVO, rat testis tissue, human liver cancer tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500-1:2000 ICC:1:50 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
