Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (C1orf96) Polyclonal Antibody, Unconjugated (ER1904-67) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-C1orf96 (ER1904-67)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30kD Observed band size: 34kD| Product Name | C1orf96 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C1orf96 FLJ37296 FLJ41471 Uncharacterized protein C1orf96 CCSAP_HUMAN Centriole, cilia and spindle-associated protein CCSAP CSAP RP4-803J11.3. |
| Product description | May play a role in embryonic development. May be required for proper cilia beating. |
| Immunogen | KLH conjugated synthetic peptide derived from human C1orf96 171-270/270 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | C1orf96
FLJ37296
FLJ41471
Uncharacterized protein C1orf96
CCSAP_HUMAN
Centriole cilia and spindle-associated protein CCSAP CSAP RP4-803J11.3. |
|---|---|
| Molecular Weight(MW) | 30 KDa |
| Cellular Localization | Cytoplasm, cytoskeleton, centrosome, centriole. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, cilium axoneme. Cell projection, axon. Cell projection, cilium. Note=Localizes to two to four centrioles throughout the cell cycle. Localizes to mitotic spindle microtubules during prometaphase and throughout the remainder of mitosis. Localizes to cytoskeleton on interphase. Localizes at the ciliary transition zone which connects the basal bodies to ciliary microtubules. Colocalizes with polyglutamylated tubulin. |
| SwissProt ID | Q6IQ19 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (C1orf96) Polyclonal Antibody, Unconjugated (ER1904-67) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-C1orf96 (ER1904-67)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30kD Observed band size: 34kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
