Application
Fig1: Sample: Pancreas (Mouse) Lysate at 40 ug Primary: Anti-C1s (ER1904-69) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47/75 kD Observed band size: 47 kDApplication
Fig2: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-C1s (ER1904-69) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47/75 kD Observed band size: 55 kDApplication
Fig3: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-C1S Polyclonal Antibody, Unconjugated(ER1904-69) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | C1s |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Basic proline rich peptide IB 1 C1 esterase C1S C1S_HUMAN Complement C1s subcomponent Complement C1s subcomponent heavy chain Complement C1s subcomponent light chain Complement component 1 s subcomponent Complement component 1 subcomponent s FLJ44757. |
| Product description | C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4. |
| Immunogen | KLH conjugated synthetic peptide derived from human C1s 251-350/688 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatHorse |
| Concentration | 1mg/ml |
| Alternative Names | Basic proline rich peptide IB 1 C1 esterase C1S C1S_HUMAN Complement C1s subcomponent Complement C1s subcomponent heavy chain Complement C1s subcomponent light chain Complement component 1 s subcomponent Complement component 1 subcomponent s FLJ44757. |
|---|---|
| Molecular Weight(MW) | 47/75 KDa |
| SwissProt ID | P09871 |
|---|
Application
Fig1: Sample: Pancreas (Mouse) Lysate at 40 ug Primary: Anti-C1s (ER1904-69) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47/75 kD Observed band size: 47 kD
Application
Fig2: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-C1s (ER1904-69) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47/75 kD Observed band size: 55 kD
Application
Fig3: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-C1S Polyclonal Antibody, Unconjugated(ER1904-69) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
