Application
Fig1: Western blot analysis of Retinoid X Receptor alpha on MCF-7 cell using anti-Retinoid X Receptor alpha antibody at 1/5,000 dilution.Application
Fig2: ICC staining Retinoid X Receptor alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Retinoid X Receptor alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Retinoid X Receptor alpha antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Retinoid X Receptor alpha antibody. Counter stained with hematoxylin.| Product Name | Retinoid X Receptor alpha [JG99-38] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ00280 antibody FLJ00318 antibody FLJ16020 antibody FLJ16733 antibody MGC102720 antibody NR2B1 antibody Nuclear receptor subfamily 2 group B member 1 antibody OTTHUMP00000022510 antibody Retinoic acid receptor RXR alpha antibody Retinoic acid receptor RXR-alpha antibody Retinoid X nuclear receptor alpha antibody Retinoid X receptor alpha antibody RXR alpha1 antibody Rxra antibody RXRA_HUMAN antibody RXRalpha1 antibody |
| Product description | Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. |
| Immunogen | Recombinant protein corresponding to N-terminal human Retinoid X Receptor alpha. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCIP |
| WB:1:1,000-1:5,000 IHC:1:50-1:200 ICC:1:50-1:200 IP:1:10-1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | FLJ00280 antibody FLJ00318 antibody FLJ16020 antibody FLJ16733 antibody MGC102720 antibody NR2B1 antibody Nuclear receptor subfamily 2 group B member 1 antibody OTTHUMP00000022510 antibody Retinoic acid receptor RXR alpha antibody Retinoic acid receptor RXR-alpha antibody Retinoid X nuclear receptor alpha antibody Retinoid X receptor alpha antibody RXR alpha1 antibody Rxra antibody RXRA_HUMAN antibody RXRalpha1 antibody |
|---|---|
| Molecular Weight(MW) | 51 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of Retinoid X Receptor alpha on MCF-7 cell using anti-Retinoid X Receptor alpha antibody at 1/5,000 dilution.
Application
Fig2: ICC staining Retinoid X Receptor alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Retinoid X Receptor alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Retinoid X Receptor alpha antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Retinoid X Receptor alpha antibody. Counter stained with hematoxylin.| Positive Control | MCF-7, rat liver tissue, human esophagus tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 IHC:1:50-1:200 ICC:1:50-1:200 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
