Application
Fig1: Western blot analysis of MGEA5 on PC-3M and SH-SY-5Y cell lysates using anti-MGEA5 antibody at 1/500 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.| Product Name | MGEA5 [JG40-05] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Beta-hexosaminidase antibody Beta-N-acetylglucosaminidase antibody Beta-N-acetylhexosaminidase antibody Bifunctional protein NCOAT antibody HEXC antibody Hyaluronidase in meningioma antibody KIAA0679 antibody MEA5 antibody Meningioma expressed antigen 5 antibody Meningioma-expressed antigen 5 antibody MGEA 5 antibody Mgea5 antibody N-acetyl-beta-D-glucosaminidase antibody N-acetyl-beta-glucosaminidase antibody NCOAT antibody Nuclear cytoplasmic O GlcNAcase and acetyltransferase antibody Nuclear cytoplasmic O-GlcNAcase and acetyltransferase antibody OGA antibody OGA_HUMAN antibody Protein O-GlcNAcase antibody |
| Product description | Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity. Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1. |
| Immunogen | Recombinant protein of N-terminal human MGEA5. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIP |
| WB:1:500-1:2,000 IHC:1:50-1:200 IP:1:10-1:50 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | Beta-hexosaminidase antibody Beta-N-acetylglucosaminidase antibody Beta-N-acetylhexosaminidase antibody Bifunctional protein NCOAT antibody HEXC antibody Hyaluronidase in meningioma antibody KIAA0679 antibody MEA5 antibody Meningioma expressed antigen 5 antibody Meningioma-expressed antigen 5 antibody MGEA 5 antibody Mgea5 antibody N-acetyl-beta-D-glucosaminidase antibody N-acetyl-beta-glucosaminidase antibody NCOAT antibody Nuclear cytoplasmic O GlcNAcase and acetyltransferase antibody Nuclear cytoplasmic O-GlcNAcase and acetyltransferase antibody OGA antibody OGA_HUMAN antibody Protein O-GlcNAcase antibody |
|---|---|
| Molecular Weight(MW) | 103 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig1: Western blot analysis of MGEA5 on PC-3M and SH-SY-5Y cell lysates using anti-MGEA5 antibody at 1/500 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.| Positive Control | SH-SY-5Y, human tonsil tissue, human pancreas tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
