Application
Fig1: Sample: 293T Cell (Human) Lysate at 30 ug Primary: Anti- C20orf31 (ER1904-74) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62kD Observed band size: 62kDApplication
Fig2: Sample:Hela Cell (Human) Lysate at 30 ug Primary: Anti- C20orf31 (ER1904-74) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62kD Observed band size: 62kDApplication
Fig3: Sample: Raji Cells Lysate at 25 ug Primary: Anti-C20orf31(ER1904-74) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62kD Observed band size: 62kD| Product Name | C20orf31 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | bA4204.1 C20orf49 EDEM 2 EDEM2 EDEM2_HUMAN ER degradation enhancer mannosidase alpha like 2 ER degradation enhancing alpha mannosidase like 2 ER degradation enhancing mannosidase like protein 2 ER degradation-enhancing alpha-mannosidase-like 2 FLJ10783 |
| Product description | Involved in endoplasmic reticulum-associated degradation (ERAD) that targets misfolded glycoproteins for degradation in an N-glycan-dependent manner. It lacks mannosidase activity. Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. |
| Immunogen | KLH conjugated synthetic peptide derived from human C20orf31 101-200/578 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | bA4204.1 C20orf49 EDEM 2 EDEM2 EDEM2_HUMAN ER degradation enhancer mannosidase alpha like 2 ER degradation enhancing alpha mannosidase like 2 ER degradation enhancing mannosidase like protein 2 ER degradation-enhancing alpha-mannosidase-like 2 FLJ10783 |
|---|---|
| Molecular Weight(MW) | 62 KDa |
| Cellular Localization | Secreted. Endoplasmic reticulum lumen. |
| SwissProt ID | Q9BV94 |
|---|
Application
Fig1: Sample: 293T Cell (Human) Lysate at 30 ug Primary: Anti- C20orf31 (ER1904-74) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62kD Observed band size: 62kD
Application
Fig2: Sample:Hela Cell (Human) Lysate at 30 ug Primary: Anti- C20orf31 (ER1904-74) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62kD Observed band size: 62kD
Application
Fig3: Sample: Raji Cells Lysate at 25 ug Primary: Anti-C20orf31(ER1904-74) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62kD Observed band size: 62kD| Positive Control | Expressed ubiquitously in all tissues tested with slightly higher levels detected in small intestine and peripheral blood leukocytes and weakest levels in brain and skeletal muscle. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
