Application
Fig1: Blank control: hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1ug in 100 uL1X PBS containing 0.5% BSA(green).Application
Fig2: Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti-C21orf33 (ER1904-78) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD| Product Name | C21orf33 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Chromosome 21 open reading frame 33 D21S2048E ES 1 ES1 Es1 protein ES1 protein homolog mitochondrial GT335 HES 1 HES1 Human HES1 protein homolog to E.coli and zebrafish ES1 protein Keio novel protein I KNP I KNPH KNPI Protein GT335 Protein KNP I ES1_HUMAN. |
| Immunogen | KLH conjugated synthetic peptide derived from human C21orf33 101-200/268 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatChickenDogPig |
| Concentration | 1mg/ml |
| Alternative Names | Chromosome 21 open reading frame 33 D21S2048E ES 1 ES1 Es1 protein ES1 protein homolog mitochondrial GT335 HES 1 HES1 Human HES1 protein homolog to E.coli and zebrafish ES1 protein Keio novel protein I KNP I KNPH KNPI Protein GT335 Protein KNP I ES1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 28 KDa |
| Cellular Localization | Mitochondrion (Potential). |
| SwissProt ID | P30042 |
|---|
Application
Fig1: Blank control: hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1ug in 100 uL1X PBS containing 0.5% BSA(green).
Application
Fig2: Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti-C21orf33 (ER1904-78) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD| Positive Control | Ubiquitous, but strongly expressed in heartand skeletal muscle. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
