Application
Fig1: Western blot analysis of VAMP1 on different tissue lysates using anti-VAMP1 antibody at 1/2,000 dilution. Positive control: Lane 1: Mouse cerebellum Lane 2: Rat brainApplication
Fig2: ICC staining VAMP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining VAMP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining VAMP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.| Product Name | VAMP1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP1 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Multiple alternative splice variants that encode proteins with alternative carboxy ends have been described, but the full-length nature of some variants has not been defined. |
| Immunogen | Recombinant full length protein of human VAMP1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICCIFIHCWB |
| WB:1:500-1:2,000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | DKFZp686H12131 antibody SYB 1 antibody SYB1 antibody Synaptobrevin 1 antibody Synaptobrevin-1 antibody Synaptobrevin1 antibody VAMP 1 antibody VAMP-1 antibody Vamp1 antibody VAMP1_HUMAN antibody Vesicle associated membrane protein 1 antibody Vesicle associated membrane protein 1 synaptobrevin 1 antibody Vesicle-associated membrane protein 1 antibody |
|---|---|
| Molecular Weight(MW) | 15kDa |
| Cellular Localization | Mitochondrion. Synapse. Synaptosome. |
Application
Fig1: Western blot analysis of VAMP1 on different tissue lysates using anti-VAMP1 antibody at 1/2,000 dilution. Positive control: Lane 1: Mouse cerebellum Lane 2: Rat brain
Application
Fig2: ICC staining VAMP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining VAMP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining VAMP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.| Positive Control | Mouse cerebellum tissue, rat brain tissue, 293T, Hela, SH-SY-5Y, human prostate cancer tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
