Application
Fig1: Sample:Placenta (Mouse) Lysate at 40 ug Primary: Anti- C22orf25 (ER1904-82)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31kD Observed band size: 35kDApplication
Fig2: Sample:Lovo Cell (Human) Lysate at 30 ug Primary: Anti- C22orf25 (ER1904-82)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31kD Observed band size: 35kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (C22orf25) Polyclonal Antibody, Unconjugated (ER1904-82) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Product Name | C22orf25 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Uncharacterized protein C22orf25 chromosome 22 open reading frame 25 DKFZp 761 P 1121 Hypothetical protein LOC128989 TNG2_HUMAN. |
| Immunogen | KLH conjugated synthetic peptide derived from human C22orf25 21-120/276 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseZebra Fish |
| Concentration | 1mg/ml |
| Alternative Names | Uncharacterized protein C22orf25 chromosome 22 open reading frame 25 DKFZp 761 P 1121 Hypothetical protein LOC128989 TNG2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 31 KDa |
| SwissProt ID | Q6ICL3 |
|---|
Application
Fig1: Sample:Placenta (Mouse) Lysate at 40 ug Primary: Anti- C22orf25 (ER1904-82)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31kD Observed band size: 35kD
Application
Fig2: Sample:Lovo Cell (Human) Lysate at 30 ug Primary: Anti- C22orf25 (ER1904-82)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31kD Observed band size: 35kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (C22orf25) Polyclonal Antibody, Unconjugated (ER1904-82) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
