Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (C3a Receptor) Polyclonal Antibody, Unconjugated (ER1904-90) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: K562(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Lung (Mouse) Lysate at 40 ug Primary: Anti-C3a Receptor (ER1904-90) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 64 kDApplication
Fig3: Sample: A549 Cell (Human) Lysate at 30 ug Primary: Anti-C3a Receptor (ER1904-90)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 60kDApplication
Fig4: Sample: 293T Cell (Human) Lysate at 30 ug Primary: Anti-C3a Receptor (ER1904-90)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 60kD| Product Name | C3a Receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AZ 3B AZ3B C3a anaphylatoxin chemotactic receptor C3A R C3A R1 C3a-R C3AR C3AR_HUMAN C3AR1 C3R1 Complement component 3 receptor 1 Complement component 3a receptor 1 HNFAG09. |
| Product description | Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. |
| Immunogen | KLH conjugated synthetic peptide derived from human C3a Receptor/C3aR 401-482/482 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | AZ 3B AZ3B C3a anaphylatoxin chemotactic receptor C3A R C3A R1 C3a-R C3AR C3AR_HUMAN C3AR1 C3R1 Complement component 3 receptor 1 Complement component 3a receptor 1 HNFAG09. |
|---|---|
| Molecular Weight(MW) | 54 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | Q16581 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (C3a Receptor) Polyclonal Antibody, Unconjugated (ER1904-90) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: K562(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Lung (Mouse) Lysate at 40 ug Primary: Anti-C3a Receptor (ER1904-90) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 64 kD
Application
Fig3: Sample: A549 Cell (Human) Lysate at 30 ug Primary: Anti-C3a Receptor (ER1904-90)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 60kD
Application
Fig4: Sample: 293T Cell (Human) Lysate at 30 ug Primary: Anti-C3a Receptor (ER1904-90)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 60kD| Positive Control | Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
