Application
Fig1: Western blot analysis of BRD2 on SiHa cell lysate using anti-BRD2 antibody at 1/2,000 dilution.Application
Fig2: ICC staining BRD2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining BRD2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining BRD2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BRD2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BRD2 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of 293T cells with BRD2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | BRD2 [JG40-42] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRD 2 antibody Brd2 antibody BRD2_HUMAN antibody Bromodomain containing 2 antibody Bromodomain-containing protein 2 antibody D6S113E antibody DKFZp686N0336 antibody Female sterile homeotic related gene 1 antibody Female sterile homeotic related gene 1, mouse, homolog of antibody FLJ31942 antibody FSH antibody FSRG1 antibody KIAA9001 antibody NAT antibody O27.1.1 antibody Really interesting new gene 3 protein antibody RING3 antibody RING3 PROTEIN antibody RNF3 antibody |
| Product description | The bromodomain-containing proteins include BRD2, BRD3, BRD4 and BRDT. BRD2 (RING3 protein) is a mitogen-activated nuclear protein whose gene is located in the human MHC II region, suggesting its relation to HLA-associated diseases. The gene encoding BRD3 (RING3-like protein) contains two bromodomains and maps to chromosome 9q34. BRD4 (HUNK1 protein) is a nuclear protein involved in the regulation of chromosomal dynamics during mitosis. The testis-specific bromodomain protein BRDT contains a PEST sequence, indicating that it undergoes rapid intracellular degradation. The bromodomain-containing proteins are ubiquitously expressed. |
| Immunogen | Recombinant protein within human BRD2 aa 1-200. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | BRD 2 antibody
Brd2 antibody
BRD2_HUMAN antibody
Bromodomain containing 2 antibody
Bromodomain-containing protein 2 antibody
D6S113E antibody
DKFZp686N0336 antibody
Female sterile homeotic related gene 1 antibody
Female sterile homeotic related gene 1 mouse homolog of antibody FLJ31942 antibody FSH antibody FSRG1 antibody KIAA9001 antibody NAT antibody O27.1.1 antibody Really interesting new gene 3 protein antibody RING3 antibody RING3 PROTEIN antibody RNF3 antibody |
|---|---|
| Molecular Weight(MW) | Predicted band size 88 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P25440 |
|---|
Application
Fig1: Western blot analysis of BRD2 on SiHa cell lysate using anti-BRD2 antibody at 1/2,000 dilution.
Application
Fig2: ICC staining BRD2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining BRD2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining BRD2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BRD2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BRD2 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of 293T cells with BRD2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | SiHa, 293T, A431, human tonsil tissue, human colon tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
