Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (C6ORF199) Polyclonal Antibody, Unconjugated (ER1905-06) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Application
Fig2: Sample:HepG2 (Human)CellLysate t 40 ug Primary: Anti-C6ORF199(ER1905-06)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 221kD Observed band size: 245kD| Product Name | C6ORF199 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | adenylate kinase domain containing 1 adenylate kinase domain containing 2 Adenylate kinase domain-containing protein 1 Adenylate kinase domain-containing protein 2 AKD1 AKD1_HUMAN AKD2 C6orf224 chromosome 6 open reading frame 199 chromosome 6 open reading frame 224 dJ70A9.1 FLJ16163 FLJ25791 FLJ34784 FLJ42177 Gm234 Gm7127 MGC126763 MGC138153 MGC177059 MGC180194 MGC184281 MGC26954 OTTHUMP00000040459 OTTHUMP00000040461 RP1-70A9. |
| Immunogen | KLH conjugated synthetic peptide derived from human C6ORF199 1601-1700/1911 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | adenylate kinase domain containing 1 adenylate kinase domain containing 2 Adenylate kinase domain-containing protein 1 Adenylate kinase domain-containing protein 2 AKD1 AKD1_HUMAN AKD2 C6orf224 chromosome 6 open reading frame 199 chromosome 6 open reading frame 224 dJ70A9.1 FLJ16163 FLJ25791 FLJ34784 FLJ42177 Gm234 Gm7127 MGC126763 MGC138153 MGC177059 MGC180194 MGC184281 MGC26954 OTTHUMP00000040459 OTTHUMP00000040461 RP1-70A9. |
|---|---|
| Molecular Weight(MW) | 221 KDa |
| SwissProt ID | Q5TCS8 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (C6ORF199) Polyclonal Antibody, Unconjugated (ER1905-06) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Application
Fig2: Sample:HepG2 (Human)CellLysate t 40 ug Primary: Anti-C6ORF199(ER1905-06)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 221kD Observed band size: 245kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
