Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Calcitonin) Polyclonal Antibody, Unconjugated (ER1905-32) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Calcitonin (ER1905-32) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 3.5/13 kD Observed band size: 17 kD| Product Name | Calcitonin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alpha CGRP CALC 1 CALC A CALC1 CALCA Calcitonin related polypeptide alpha CGRP 1 CGRP CGRP I CGRP-1 CGRPI CGRP1 CT Katacalcin KC MGC126648 alpha type CGRP calcitonin 1 calcitonin gene related peptide 1 calcitonin gene related peptide I calcitonin isoform CT preproprotein. |
| Product description | Calcitonin is a 32 amino acid peptide hormone secreted by parafollicular cells (also known as C cells) of the thyroid gland in humans, and in many other animals in the ultimopharyngeal body. It acts to reduce blood calcium (Ca2+), opposing the effects of parathyroid hormone (PTH). Calcitonin has been found in fish, reptiles, birds, and mammals. Its importance in humans has not been as well established as its importance in other animals, as its function is usually not significant in the regulation of normal calcium homeostasis. It belongs to the calcitonin-like protein family. The hormone participates in calcium (Ca2+) and phosphorus metabolism. In many ways, calcitonin counteracts parathyroid hormone (PTH) and vitamin D. High concentrations of calcitonin may be able to increase urinary excretion of calcium and phosphate via the renal tubules.[11] leading to marked hypocalcemia. However, this is a minor effect with no physiological significance in humans. It is also a short-lived effect because the kidneys become resistant to calcitonin, as demonstrated by the kidney's unaffected excretion of calcium in patients with thyroid tumors that secrete excessive calcitonin. |
| Immunogen | KLH conjugated synthetic peptide derived from human Calcitonin 85-116/141 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogRabbitGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | Alpha CGRP
CALC 1
CALC A CALC1 CALCA Calcitonin related polypeptide alpha CGRP 1 CGRP CGRP I CGRP-1 CGRPI CGRP1 CT Katacalcin KC MGC126648 alpha type CGRP calcitonin 1 calcitonin gene related peptide 1 calcitonin gene related peptide I calcitonin isoform CT preproprotein. |
|---|---|
| Molecular Weight(MW) | 3.5/13 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P01258 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Calcitonin) Polyclonal Antibody, Unconjugated (ER1905-32) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Calcitonin (ER1905-32) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 3.5/13 kD Observed band size: 17 kD| Positive Control | Mouse brain tissue, Cerebrum (Mouse) Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
