Application
Fig1: Western blot analysis of IMPDH2 on Daudi cell lysate using anti-IMPDH2 antibody at 1/2,000 dilution.Application
Fig2: ICC staining IMPDH2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining IMPDH2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining IMPDH2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of Daudi cells with IMPDH2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | IMPDH2 [JE40-87] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IMDH2_HUMAN antibody IMP (inosine monophosphate) dehydrogenase 2 antibody IMP dehydrogenase 2 antibody IMP oxireductase 2 antibody IMPD 2 antibody IMPD2 antibody IMPDH 2 antibody IMPDH II antibody IMPDH-II antibody Impdh2 antibody Inosine 5' monophosphate dehydrogenase 2 antibody Inosine 5' phosphate dehydrogenase 2 antibody Inosine monophosphate dehydrogenase type II antibody Inosine-5''-monophosphate dehydrogenase 2 antibody |
| Product description | Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors. |
| Immunogen | Recombinant protein within C-terminal human IMPDH2. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFCIP |
| WB:1:500-1:2,000 ICC/IF:1:50-1:200 IHC:1:50-1:200 FC:1:10-1:50 IP:1:10-1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | IMDH2_HUMAN antibody IMP (inosine monophosphate) dehydrogenase 2 antibody IMP dehydrogenase 2 antibody IMP oxireductase 2 antibody IMPD 2 antibody IMPD2 antibody IMPDH 2 antibody IMPDH II antibody IMPDH-II antibody Impdh2 antibody Inosine 5' monophosphate dehydrogenase 2 antibody Inosine 5' phosphate dehydrogenase 2 antibody Inosine monophosphate dehydrogenase type II antibody Inosine-5''-monophosphate dehydrogenase 2 antibody |
|---|---|
| Molecular Weight(MW) | 56 kDa |
| Cellular Localization | Nucleus. Cytoplasm. |
Application
Fig1: Western blot analysis of IMPDH2 on Daudi cell lysate using anti-IMPDH2 antibody at 1/2,000 dilution.
Application
Fig2: ICC staining IMPDH2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining IMPDH2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining IMPDH2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-IMPDH2 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of Daudi cells with IMPDH2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Daudi, A431, LOVO, PC-3M, rat kidney tissue, human tonsil tissue, human thyroid gland tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC/IF:1:50-1:200 IHC:1:50-1:200 FC:1:10-1:50 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
