Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (CAMKK2) Polyclonal Antibody, Unconjugated (ER1905-35) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Application
Fig2: Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti-CAMKK2 (ER1905-35) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kDApplication
Fig3: Blank control (Black line): Molt4 (Black). Primary Antibody (green line):Rabbit Anti-CAMKK2 antibody (ER1905-35) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig4: Molt-4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CAMKK2 Antibody(ER1905-35)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).Application
Fig5: Sample: Cerebral cortex (Mouse) Lysate at 40 ug Cerebral cortex (Rat) Lysate at 40 ug Primary: Anti-CAMKK2 (ER1905-35) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD| Product Name | CAMKK2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Calcium/calmodulin dependent protein kinase beta Calcium/calmodulin dependent protein kinase kinase 2 Calcium/calmodulin dependent protein kinase kinase beta CaM kinase kinase beta CaM KK beta CAMKK 2 CAMKK CaMKK beta CAMKK beta protein CAMKKB. |
| Product description | Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Efficiently phosphorylates 5'-AMP-activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals (By similarity). Seems to be involved in hippocampal activation of CREB1 (By similarity). May play a role in neurite growth. Isoform 3 may promote neurite elongation, while isoform 1 may promoter neurite branching. |
| Immunogen | KLH conjugated synthetic peptide derived from human CAMKK2 501-588/588 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1ug/Test: |
|
| Species Reactivity | HumanMouseRatPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Calcium/calmodulin dependent protein kinase beta Calcium/calmodulin dependent protein kinase kinase 2 Calcium/calmodulin dependent protein kinase kinase beta CaM kinase kinase beta CaM KK beta CAMKK 2 CAMKK CaMKK beta CAMKK beta protein CAMKKB. |
|---|---|
| Molecular Weight(MW) | 55 KDa |
| Cellular Localization | Nucleus. Cytoplasm. Cell projection. Note=Predominantly nuclear in unstimulated cells. Found in the cytoplasm and neurites after forskolin induction. |
| SwissProt ID | Q96RR4 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (CAMKK2) Polyclonal Antibody, Unconjugated (ER1905-35) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Application
Fig2: Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti-CAMKK2 (ER1905-35) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD
Application
Fig3: Blank control (Black line): Molt4 (Black). Primary Antibody (green line):Rabbit Anti-CAMKK2 antibody (ER1905-35) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig4: Molt-4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CAMKK2 Antibody(ER1905-35)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Application
Fig5: Sample: Cerebral cortex (Mouse) Lysate at 40 ug Cerebral cortex (Rat) Lysate at 40 ug Primary: Anti-CAMKK2 (ER1905-35) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD| Positive Control | Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
