Application
Fig1: Sample:
U87MG(Human) Cell Lysate at 30 ug
U2OS(Human) Cell Lysate at 30 ug
Primary: Anti- CAPRIN2 (ER1905-37) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 126 kD
Observed band size: 126 kD
Application
r 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CAPRIN2) Polyclonal Antibody, Unconjugated (ER1905-37) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.