Application
Fig1: Sample: Jurkat(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug Primary: Anti-CARD14 (ER1905-39) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 113 kD Observed band size: 113 kDApplication
Fig2: Sample: Fetal brain (Mouse) Lysate at 40 ug Primary: Anti-CARD14 (ER1905-39) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 113 kD Observed band size: 113 kDApplication
Fig3: Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-CARD14 (ER1905-39) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 113 kD Observed band size: 113 kD| Product Name | CARD14 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Bcl10 interacting MAGUK protein 2 Bimp 2 Bimp2 CARD 14 CARD containing MAGUK 2 protein CARD containing MAGUK protein 2 Card maguk protein 2 Carma 2 Carma2 Caspase recruitment domain containing protein 14 Caspase recruitment domain family member 14 Caspase recruitment domain protein 14 CAR14_HUMAN. |
| Product description | Plays a role in signaling mediated by TRAF2, TRAF3 and TRAF6 and protects cells against apoptosis. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10. |
| Immunogen | KLH conjugated synthetic peptide derived from human CARD14 1-100/1004 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Bcl10 interacting MAGUK protein 2 Bimp 2 Bimp2 CARD 14 CARD containing MAGUK 2 protein CARD containing MAGUK protein 2 Card maguk protein 2 Carma 2 Carma2 Caspase recruitment domain containing protein 14 Caspase recruitment domain family member 14 Caspase recruitment domain protein 14 CAR14_HUMAN. |
|---|---|
| Molecular Weight(MW) | 113 KDa |
| Cellular Localization | Isoform 1: Cytoplasm.;Isoform 2: Cytoplasm.;Isoform 3: Cytoplasm. |
| SwissProt ID | Q9BXL6 |
|---|
Application
Fig1: Sample: Jurkat(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug Primary: Anti-CARD14 (ER1905-39) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 113 kD Observed band size: 113 kD
Application
Fig2: Sample: Fetal brain (Mouse) Lysate at 40 ug Primary: Anti-CARD14 (ER1905-39) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 113 kD Observed band size: 113 kD
Application
Fig3: Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-CARD14 (ER1905-39) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 113 kD Observed band size: 113 kD| Positive Control | Isoform 1 is detected in placenta and epidermal keratinocytes. Isoform 2 is detected in leukocytes and fetal brain. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
