Application
Fig1: Sample: MCF-7(Human) Cell Lysate at 30 ug Primary: Anti-NOD2 (ER1905-40) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 114 kD Observed band size: 114 kDApplication
Fig2: Sample: A549(Human) Cell Lysate at 30 ug MCF-7(Human) Cell Lysate at 30 ug Primary: Anti-NOD2 (ER1905-40) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 114 kD Observed band size: 114 kDApplication
Fig3: Blank control(blue): Raji(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-CARD15/AF488 Conjugated antibody(ER1905-40-/AF488 ), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions );| Product Name | CARD15/NOD2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACUG Arthrocutaneouveal granulomatosis BLAU CARD15 Caspase recruitment domain protein 15 Caspase recruitment domain-containing protein 15 CD IBD1 Inflammatory bowel disease protein 1 LRR containing protein LRR-containing protein Nod2 NOD2 protein NOD2_HUMAN NOD2B nucleotide binding oligomerization domain 2 nucleotide-binding oligomerization domain 2 Nucleotide-binding oligomerization domain-containing protein 2 PSORAS1. |
| Immunogen | KLH conjugated synthetic peptide derived from human CARD15 251-350/1040 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1ug/Test: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | ACUG Arthrocutaneouveal granulomatosis BLAU CARD15 Caspase recruitment domain protein 15 Caspase recruitment domain-containing protein 15 CD IBD1 Inflammatory bowel disease protein 1 LRR containing protein LRR-containing protein Nod2 NOD2 protein NOD2_HUMAN NOD2B nucleotide binding oligomerization domain 2 nucleotide-binding oligomerization domain 2 Nucleotide-binding oligomerization domain-containing protein 2 PSORAS1. |
|---|---|
| Molecular Weight(MW) | 114 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q9HC29 |
|---|
Application
Fig1: Sample: MCF-7(Human) Cell Lysate at 30 ug Primary: Anti-NOD2 (ER1905-40) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 114 kD Observed band size: 114 kD
Application
Fig2: Sample: A549(Human) Cell Lysate at 30 ug MCF-7(Human) Cell Lysate at 30 ug Primary: Anti-NOD2 (ER1905-40) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 114 kD Observed band size: 114 kD
Application
Fig3: Blank control(blue): Raji(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-CARD15/AF488 Conjugated antibody(ER1905-40-/AF488 ), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions );| Positive Control | Monocytes-specific. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
