Application
Fig1: Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti- CASC5 (ER1905-44) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 265 kD Observed band size: 265 kD| Product Name | CASC5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein D40/AF15q14 AF15Q14 ALL1 fused gene from chromosome 15q14 Blinkin bub linking kinetochore protein Cancer susceptibility candidate 5 Cancer susceptibility candidate gene 5 protein Cancer/testis antigen 29 CT29 D40 hKNL 1 hSpc105 KIAA1570 Kinetochore null 1 homolog Kinetochore null protein 1 KNL1 CASC5_HUMAN. |
| Product description | Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Required for attachment of the kinetochores to the spindle microtubules. Directly links BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore. |
| Immunogen | KLH conjugated synthetic peptide derived from human CASC5 2251-2342/2342 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Protein D40/AF15q14 AF15Q14 ALL1 fused gene from chromosome 15q14 Blinkin bub linking kinetochore protein Cancer susceptibility candidate 5 Cancer susceptibility candidate gene 5 protein Cancer/testis antigen 29 CT29 D40 hKNL 1 hSpc105 KIAA1570 Kinetochore null 1 homolog Kinetochore null protein 1 KNL1 CASC5_HUMAN. |
|---|---|
| Molecular Weight(MW) | 265 KDa |
| Cellular Localization | Nucleus. Chromosome, centromere, kinetochore. |
| SwissProt ID | Q8NG31 |
|---|
Application
Fig1: Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti- CASC5 (ER1905-44) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 265 kD Observed band size: 265 kD| Positive Control | Highly expressed in testis, where it is localized in germ cells, in particular in spermatocytes and in the pre-acrosome of round spermatids. Detected in the acrosome of ejaculated spermatozoa. Detected in adult thymus, bone marrow, colon, small intestine, appendix and placenta, and in fetal liver and thymus. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
