Application
Fig1: Sample:lung (rat) Lysate at 40 ug Primary: Anti-Caspase-1 p20(ER1905-47)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20/46kD Observed band size: 46kDApplication
Fig2: Sample:large intestine (rat) Lysate at 40 ug Primary: Anti-Caspase-1 p20(ER1905-47)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20/46kD Observed band size: 46kDApplication
Fig3: Blank control (blue line): MCF7(fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice) Primary Antibody (green line): Rabbit Anti-Caspase-1 p20 antibody (ER1905-47), Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC| Product Name | Caspase-1 p20 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | caspase-1 isoform alpha p20 subunit CASP1 ICE IL1BC Caspase-1 subunit p20 CASP 1 Caspase 1 apoptosis related cysteine peptidase Caspase 1 apoptosis related cysteine protease Caspase1 IL 1 beta converting enzyme IL 1BC IL1B convertase IL1BCE Interleukin 1 beta convertase Interleukin 1 beta convertase precursor Interleukin 1 beta converting enzyme CASP-1 Interleukin-1 beta convertase IL-1BC Interleukin-1 beta-converting enzyme ICE IL-1 beta-converting enzyme p45 |
| Product description | Caspase-1/Interleukin-1 converting enzyme (ICE) is an evolutionarily conserved enzyme that proteolytically cleaves other proteins, such as the precursors of the inflammatory cytokines interleukin 1β and interleukin 18 as well as the pyroptosis inducer Gasdermin D, into active mature peptides. It plays a central role in cell immunity as an inflammatory response initiator. Once activated through formation of an inflammasome complex, it initiates a proinflammatory response through the cleavage and thus activation of the two inflammatory cytokines, interleukin 1β (IL-1β) and interleukin 18 (IL-18) as well as pyroptosis, a programmed lytic cell death pathway, through cleavage of Gasdermin D. The two inflammatory cytokines activated by Caspase-1 are excreted from the cell to further induce the inflammatory response in neighboring cells. Activated Caspase 1 proteolytically cleaves pro IL-1β and pro IL-18 into their active forms, IL-1β and IL-18. The active cytokines lead to a downstream inflammatory response. It also cleaves Gasdermin D into its active form, which leads to pyroptosis. Once matured, the cytokines initiate downstream signaling events to induce a proinflammatory response as well as to activate the expression of antiviral genes. Following the inflammatory response, an activated Caspase-1 can induce pyroptosis, a lytic form of cell death, depending on the signal received as well as the specific inflammasome sensor domain protein that received it. Caspase-1 has also been shown to induce necrosis and may also function in various developmental stages. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse Caspase-1 p20 181-280/402 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | caspase-1 isoform alpha p20 subunit
CASP1
ICE IL1BC Caspase-1 subunit p20 CASP 1 Caspase 1 apoptosis related cysteine peptidase Caspase 1 apoptosis related cysteine protease Caspase1 IL 1 beta converting enzyme IL 1BC IL1B convertase IL1BCE Interleukin 1 beta convertase Interleukin 1 beta convertase precursor Interleukin 1 beta converting enzyme CASP-1 Interleukin-1 beta convertase IL-1BC Interleukin-1 beta-converting enzyme ICE IL-1 beta-converting enzyme p45 |
|---|---|
| Molecular Weight(MW) | 20/46 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P29466 |
|---|
Application
Fig1: Sample:lung (rat) Lysate at 40 ug Primary: Anti-Caspase-1 p20(ER1905-47)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20/46kD Observed band size: 46kD
Application
Fig2: Sample:large intestine (rat) Lysate at 40 ug Primary: Anti-Caspase-1 p20(ER1905-47)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20/46kD Observed band size: 46kD
Application
Fig3: Blank control (blue line): MCF7(fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice) Primary Antibody (green line): Rabbit Anti-Caspase-1 p20 antibody (ER1905-47), Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC| Positive Control | lung (rat) Lysate, large intestine (rat) Lysate, MCF7 cell. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 |
