Application
Fig1: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-Cathepsin B (ER1905-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 32 kDApplication
Fig2: Blank control: RSC96(blue). Primary Antibody:Rabbit Anti- Cathepsin B antibody(ER1905-48), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Antibody (ER1905-48, 5μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of ER1905-48 at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.| Product Name | Cathepsin B |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Amyloid precursor protein secretase APP secretase APPS Cathepsin B CB Cathepsin B1 CathepsinB CPSB CTSB Cysteine protease Preprocathepsin B Cathepsin B heavy chain Cathepsin B light chain CATB_HUMAN. |
| Product description | Cathepsin B is synthesized on the rough endoplasmic reticulum as a preproenzyme of 339 amino acid with a signal peptide of 17 amino acids. Procathepsin B of 43/46 kDa is then transported to the Golgi apparatus and cathepsin B is formed. Mature cathepsin B is composed of a heavy chain of 25-26 kDa and a light chain of 5kDa, which are linked by a dimer of disulfide. Cathepsin B may enhance the activity of other protease, including matrix metalloproteinase, urokinase (serine protease urokinase plasminogen activator), and cathepsin D, and thus it has an essential position for in the proteolysis of extracellular matrix components, intercellular communication disruption, and reduced protease inhibitor expression. It is also involved in autophagy and catabolism, which is advantageous in tumor malignancy and is possibly involved in specific immune resistance. |
| Immunogen | KLH conjugated synthetic peptide derived from human Cathepsin B heavy chain 251-339/339 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Amyloid precursor protein secretase APP secretase APPS Cathepsin B CB Cathepsin B1 CathepsinB CPSB CTSB Cysteine protease Preprocathepsin B Cathepsin B heavy chain Cathepsin B light chain CATB_HUMAN. |
|---|---|
| Molecular Weight(MW) | 23/28/37 KDa |
| Cellular Localization | Lysosome. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| SwissProt ID | P10605 |
|---|
Application
Fig1: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-Cathepsin B (ER1905-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 32 kD
Application
Fig2: Blank control: RSC96(blue). Primary Antibody:Rabbit Anti- Cathepsin B antibody(ER1905-48), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Antibody (ER1905-48, 5μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of ER1905-48 at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.| Positive Control | Human esophageal carcinoma, Mouse Spleen Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
