Application
Fig1: Sample: A549 Cell (Human) Lysate at 30 ug K562 Cell (Human) Lysate at 30 ug Primary: Anti- Cathepsin L (ER1905-49) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19/30/37 kD Observed band size: 40/63 kDApplication
Fig2: Protein: line1, rat brain lysates, 30ug; line2, rat liver lysates, 30ug; Primary: Anti-Cathepsin L (ER1905-49) at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 37kD Observed band size : 19kD, 37kDApplication
Fig3: Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cathepsin Polyclonal Antibody, Unconjugated(ER1905-49) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig4: Sample: D5-16 Cell (Human) Lysate at 30 ug D5-23 Cell (Human) Lysate at 40 ug Primary: Anti- Cathepsin L (ER1905-49) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19/30/37 kD Observed band size: 40/63 kD| Product Name | Cathepsin L |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cathepsin L heavy chain Cathepsin L light chain CathepsinL CATL CTSL CTSL protein DKFZp686A18159 FLJ31037 Major excreted protein MEP cath-L CL CATL1_HUMAN CathepsinL1 Cathepsin L1,Cathepsin-L1 CTSL 1 CTSL-1,CTS L1,CTS-L1. |
| Product description | Cathepsin L1 is a protein that in humans is encoded by the CTSL1 gene. The protein encoded by this gene is a lysosomal cysteine protease that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene. |
| Immunogen | KLH conjugated synthetic peptide derived from human cathepsin L1 proprotein 71-170/334 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Cathepsin L heavy chain
Cathepsin L light chain
CathepsinL
CATL
CTSL
CTSL protein
DKFZp686A18159
FLJ31037
Major excreted protein
MEP
cath-L
CL
CATL1_HUMAN
CathepsinL1
Cathepsin L1 Cathepsin-L1 CTSL 1 CTSL-1 CTS L1 CTS-L1. |
|---|---|
| Molecular Weight(MW) | 19/30/37 KDa |
| Cellular Localization | Lysosome. |
| SwissProt ID | P06797 |
|---|
Application
Fig1: Sample: A549 Cell (Human) Lysate at 30 ug K562 Cell (Human) Lysate at 30 ug Primary: Anti- Cathepsin L (ER1905-49) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19/30/37 kD Observed band size: 40/63 kD
Application
Fig2: Protein: line1, rat brain lysates, 30ug; line2, rat liver lysates, 30ug; Primary: Anti-Cathepsin L (ER1905-49) at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 37kD Observed band size : 19kD, 37kD
Application
Fig3: Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cathepsin Polyclonal Antibody, Unconjugated(ER1905-49) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig4: Sample: D5-16 Cell (Human) Lysate at 30 ug D5-23 Cell (Human) Lysate at 40 ug Primary: Anti- Cathepsin L (ER1905-49) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19/30/37 kD Observed band size: 40/63 kD| Positive Control | A549 Cell Lysate, K562 Cell Lysate, rat pancreas tissue, rat brain lysates, human cervical carcinoma, D5-16 Cell Lysate, D5-23 Cell Lysate |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
