Application
Fig1: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-CATSPER (ER1905-50) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 90 kD Observed band size: 85 kD| Product Name | CATSPER |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ation channel sperm associated 1 Cation channel sperm associated protein 1 CATSPER 1 CATSPER hCatSper MGC33335 MGC33368 Sperm associated cation channel 1 Sperm ion channel CTSR1_HUMAN Cation channel sperm-associated protein 1 CatSper1 hCatSper. |
| Product description | Voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte. Activated by extracellular progesterone and prostaglandins following the sequence: progesterone > PGF1-alpha = PGE1 > PGA1 > PGE2 >> PGD2. The primary effect of progesterone activation is to shift voltage dependence towards more physiological, negative membrane potentials; it is not mediated by metabotropic receptors and second messengers. Sperm capacitation enhances the effect of progesterone by providing additional negative shift. Also activated by the elevation of intracellular pH. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse CATSPER 451-550/686 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ation channel sperm associated 1 Cation channel sperm associated protein 1 CATSPER 1 CATSPER hCatSper MGC33335 MGC33368 Sperm associated cation channel 1 Sperm ion channel CTSR1_HUMAN Cation channel sperm-associated protein 1 CatSper1 hCatSper. |
|---|---|
| Molecular Weight(MW) | 90 KDa |
| Cellular Localization | Cell projection, cilium, flagellum membrane; Multi-pass membrane protein. Note=Specifically located in the principal piece of sperm tail. |
| SwissProt ID | Q91ZR5 |
|---|
Application
Fig1: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-CATSPER (ER1905-50) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 90 kD Observed band size: 85 kD| Positive Control | Testis-specific. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
