Application
Fig1: Sample: Testis(Mouse) Cell Lysate at 40 ug Hepg2(Human) Cell Lysate at 30 ug Primary: Anti-CBX4 (ER1905-52) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kDApplication
Fig2: Sample: Adrenal gland (Mouse) Lysate at 40 ug Jurkat(Human) Cell Lysate at 30 ug Testis (Mouse) Lysate at 40 ug HepG2(Human) Cell Lysate at 30 ug Primary: Anti- CBX4 (ER1905-52) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 63 kDApplication
Fig3: Sample: K562(Human) Cell Lysate at 30 ug MCF-7(Human) Cell Lysate at 30 ug Primary: Anti- CBX4 (ER1905-52) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 61 kD| Product Name | CBX4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CBX 4 CBX4 Cbx4 chromobox homolog 4 (Drosophila Pc class) CBX4_HUMAN Chromobox homolog 4 (Pc class homolog, Drosophila) Chromobox homolog 4 Chromobox protein homolog 4 E3 SUMO protein ligase CBX 4 E3 SUMO protein ligase CBX4 E3 SUMO-protein ligase CBX4 hPc 2 hPc2 NBP 16 NBP16 NS5ATP1 binding protein 16 Pc 2 Pc class 2 homolog Pc class homolog Pc class homolog Drosophila PC2 Polycomb 2 homolog. |
| Product description | E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. |
| Immunogen | KLH conjugated synthetic peptide derived from human CBX4 401-500/560 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | CBX 4
CBX4
Cbx4 chromobox homolog 4 (Drosophila Pc class)
CBX4_HUMAN
Chromobox homolog 4 (Pc class homolog Drosophila) Chromobox homolog 4 Chromobox protein homolog 4 E3 SUMO protein ligase CBX 4 E3 SUMO protein ligase CBX4 E3 SUMO-protein ligase CBX4 hPc 2 hPc2 NBP 16 NBP16 NS5ATP1 binding protein 16 Pc 2 Pc class 2 homolog Pc class homolog Pc class homolog Drosophila PC2 Polycomb 2 homolog. |
|---|---|
| Molecular Weight(MW) | 61 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | O00257 |
|---|
Application
Fig1: Sample: Testis(Mouse) Cell Lysate at 40 ug Hepg2(Human) Cell Lysate at 30 ug Primary: Anti-CBX4 (ER1905-52) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD
Application
Fig2: Sample: Adrenal gland (Mouse) Lysate at 40 ug Jurkat(Human) Cell Lysate at 30 ug Testis (Mouse) Lysate at 40 ug HepG2(Human) Cell Lysate at 30 ug Primary: Anti- CBX4 (ER1905-52) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 63 kD
Application
Fig3: Sample: K562(Human) Cell Lysate at 30 ug MCF-7(Human) Cell Lysate at 30 ug Primary: Anti- CBX4 (ER1905-52) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 61 kD| Positive Control | Ubiquitous. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
