Application
Fig1: Western blot analysis of PEN2 on mouse spleen tissue lysate using anti-PEN2 antibody at 1/2,000 dilution.Application
Fig2: ICC staining PEN2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining PEN2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PEN2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PEN2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-PEN2 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PEN2 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PEN2 antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of A549 cells with PEN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | PEN2 [JE41-28] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gamma secretase subunit PEN 2 antibody Gamma Secretase Subunit PEN2 antibody Gamma-secretase subunit PEN-2 antibody Hematopoietic stem/progenitor cells protein MDS033 antibody MDS033 antibody MSTP064 antibody PEN 2 antibody PEN2_HUMAN antibody Presenilin Enhancer 2 antibody Presenilin enhancer 2 homolog antibody Presenilin enhancer protein 2 antibody PSEN2 antibody psenen antibody |
| Product description | Four proteins comprise the gamma-secretase complex: presenilin, nicastrin, aph-1, and PEN-2. Together, these proteins mediate cell surface signaling pathways for a variety of type I membrane proteins, notably amyloid-beta precursor protein, a protein implicated in the development of Alzheimer's disease, via intramembrane proteolysis. The proteins assemble into a proteolytically active complex in the golgi/trans-golgi network (TGN) compartments. Assembly leads to autocleavage of presenilin into two subunits to create the active site of gamma-secretase, an important step in understanding the mechanisms involved in the etiology and possible treatment of Alzheimer's disease. |
| Immunogen | Synthetic peptide corresponding to C-terminal human PEN2. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Gamma secretase subunit PEN 2 antibody Gamma Secretase Subunit PEN2 antibody Gamma-secretase subunit PEN-2 antibody Hematopoietic stem/progenitor cells protein MDS033 antibody MDS033 antibody MSTP064 antibody PEN 2 antibody PEN2_HUMAN antibody Presenilin Enhancer 2 antibody Presenilin enhancer 2 homolog antibody Presenilin enhancer protein 2 antibody PSEN2 antibody psenen antibody |
|---|---|
| Molecular Weight(MW) | 12 kDa |
| Cellular Localization | Cell membrane. Endoplasmic reticulum. Golgi apparatus. |
Application
Fig1: Western blot analysis of PEN2 on mouse spleen tissue lysate using anti-PEN2 antibody at 1/2,000 dilution.
Application
Fig2: ICC staining PEN2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PEN2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PEN2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PEN2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-PEN2 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PEN2 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PEN2 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of A549 cells with PEN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Mouse spleen tissue, rat cerebellum tissue, human tonsil tissue, human lung cancer tissue, human kidney tissue, mouse testis tissue, A549, SH-SY-5Y. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
