Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Primary: Anti- CCKBR (ER1905-69) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kDApplication
Fig2: Sample: Stomach(Mouse) Lysate at 40 ug Primary: Anti- CCKBR (ER1905-69) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 53 kD| Product Name | CCKBR |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gastrin receptor GASR CCK B CCK B receptor CCK BR CCK-B receptor CCK-BR CCK2 R CCK2 receptor CCK2-R CCKB CCKB receptor CCKBR CCKR Type B CCKRB Cholecystokinin 2 receptor Cholecystokinin B receptor Cholecystokinin-2 receptor Cholecystokinin2 receptor CholecystokininB receptor GASR GASR_HUMAN Gastrin receptor Gastrin/cholecystokinin type B receptor Gastrincholecystokinin brain receptor. |
| Product description | Receptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.;Isoform 2 is constitutively activated and may regulate cancer cell proliferation via a gastrin-independent mechanism. |
| Immunogen | KLH conjugated synthetic peptide derived from human Gastrin receptor 301-400/447 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Gastrin receptor GASR CCK B CCK B receptor CCK BR CCK-B receptor CCK-BR CCK2 R CCK2 receptor CCK2-R CCKB CCKB receptor CCKBR CCKR Type B CCKRB Cholecystokinin 2 receptor Cholecystokinin B receptor Cholecystokinin-2 receptor Cholecystokinin2 receptor CholecystokininB receptor GASR GASR_HUMAN Gastrin receptor Gastrin/cholecystokinin type B receptor Gastrincholecystokinin brain receptor. |
|---|---|
| Molecular Weight(MW) | 48 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P32239 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Primary: Anti- CCKBR (ER1905-69) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD
Application
Fig2: Sample: Stomach(Mouse) Lysate at 40 ug Primary: Anti- CCKBR (ER1905-69) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 53 kD| Positive Control | Isoform 1 is expressed in brain, pancreas, stomach, the colon cancer cell line LoVo and the T-lymphoblastoma Jurkat, but not in heart, placenta, liver, lung, skeletal muscle, kidney or the stomach cancer cell line AGS. Expressed at high levels in the small cell lung cancer cell line NCI-H510, at lower levels in NCI-H345, NCI-H69 and GLC-28 cell lines, not expressed in GLC-19 cell line. Within the stomach, expressed at high levels in the mucosa of the gastric fundus and at low levels in the antrum and duodenum. Isoform 2 is present in pancreatic cancer cells and colorectal cancer cells, but not in normal pancreas or colonic mucosa. Isoform 3 is expressed in brain, pancreas, stomach, the stomach cancer cell line AGS and the colon cancer cell line LoVo. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
