Application
Fig1: Sample: U251 Cell Lysate at 40 ug Primary: Anti-CCN1 (ER1905-70) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 48 kDApplication
Fig2: Sample: DU145(Human) Cell Lysate at 30 ug Primary: Anti- CCN1 (ER1905-70) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD| Product Name | CCN1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cyr61 IGFBP10 cysteine rich protein 61 3CH61 CCN 1 CCN1 CYR 61 CYR61 CYR61 protein CYR61 protein precursor Cysteine rich angiogenic inducer 61 Cysteine rich heparin binding protein 61 GIG 1 GIG1 GIG1 Igfbp 10 IGFBP10 Insulin like growth factor binding protein 10 Protein CYR61 Protein GIG1 AI325051 cysteine rich 61 CYR61_HUMAN . |
| Product description | Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. |
| Immunogen | KLH conjugated synthetic peptide derived from human Cyr61 251-379/379 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Cyr61 IGFBP10 cysteine rich protein 61 3CH61 CCN 1 CCN1 CYR 61 CYR61 CYR61 protein CYR61 protein precursor Cysteine rich angiogenic inducer 61 Cysteine rich heparin binding protein 61 GIG 1 GIG1 GIG1 Igfbp 10 IGFBP10 Insulin like growth factor binding protein 10 Protein CYR61 Protein GIG1 AI325051 cysteine rich 61 CYR61_HUMAN . |
|---|---|
| Molecular Weight(MW) | 42 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | O00622 |
|---|
Application
Fig1: Sample: U251 Cell Lysate at 40 ug Primary: Anti-CCN1 (ER1905-70) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 48 kD
Application
Fig2: Sample: DU145(Human) Cell Lysate at 30 ug Primary: Anti- CCN1 (ER1905-70) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
