Application
Fig1: Protein: HepG2(human) lysates at 30ug; Primary: rabbit Anti-CD101 (ER1905-74) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:113 kD Observed band size:113 kDApplication
Fig2: Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-CD101 antibody(ER1905-74), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.| Product Name | CD101 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IGSF2 Immunoglobulin superfamily member 2 Leukocyte surface protein V7 V7 LSB IGSF2_HUMAN IgSF2 Cell surface glycoprotein V7 Glu-Trp-Ile EWI motif-containing protein 101 EWI-101 CD101. |
| Product description | Plays a role as inhibitor of T-cells proliferation induced by CD3. Inhibits expression of IL2RA on activated T-cells and secretion of IL2. Inhibits tyrosine kinases that are required for IL2 production and cellular proliferation. Inhibits phospholipase C-gamma-1/PLCG1 phosphorylation and subsequent CD3-induced changes in intracellular free calcium. Prevents nuclear translocation of nuclear factor of activated T-cell to the nucleus. Plays a role in the inhibition of T-cell proliferation via IL10 secretion by cutaneous dendritic cells. May be a marker of CD4(+) CD56(+) leukemic tumor cells. |
| Immunogen | KLH conjugated synthetic peptide derived from human CD101 51-150/1021 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICC/IFFC |
| WB:1:500-2000: FC:1μg/Test: IF:1:10-100: ICC:1:100-500: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | IGSF2 Immunoglobulin superfamily member 2 Leukocyte surface protein V7 V7 LSB IGSF2_HUMAN IgSF2 Cell surface glycoprotein V7 Glu-Trp-Ile EWI motif-containing protein 101 EWI-101 CD101. |
|---|---|
| Molecular Weight(MW) | 113 KDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein (Potential). |
| SwissProt ID | Q93033 |
|---|
Application
Fig1: Protein: HepG2(human) lysates at 30ug; Primary: rabbit Anti-CD101 (ER1905-74) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:113 kD Observed band size:113 kD
Application
Fig2: Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-CD101 antibody(ER1905-74), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.| Positive Control | Expressed in lung, thymus and small intestine. Detected in cutaneous dendritic cells, activated T-cells, monocytes and granulocytes as well as with epithelial cells with dendritic morphology. Expressed in some leukemic cells, the CD4(+) CD56(+) blastic tumor cells, as well as in Langerhans cells from LCH (Langerhans cell histiocytosis) patients. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: IF:1:10-100: ICC:1:100-500: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
