Application
Fig1: Sample: Lane 1: Brain (Mouse) Lysate at 30 ug Lane 2: Lung (Mouse) Lysate at 30 ug Primary: Anti-Sema7A/CD108(ER1905-75) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 66kD Observed band size : 66kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CD108) Polyclonal Antibody, Unconjugated (ER1905-75) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Sample: Cerebrum (Mouse) Lysate at 40 ug Lung (Mouse) Lysate at 40 ug Primary: Anti-CD108 (ER1905-75) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66 kD Observed band size: 63 kDApplication
Fig4: Sample: Skin (Mouse) Lysate at 40 ug Skin(Rat) Lysate at 40 ug Primary: Anti-CD108 (ER1905-75) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66 kD Observed band size: 66 kD| Product Name | CD108 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | semaphorin 7A CD 108 CD108 CD108 antigen CDw108 H Sema K1 H Sema L JMH JMH blood group antigen John Milton Hagen blood group H Sema K1 John Milton Hargen human blood group Ag MGC126692 MGC126696 Sema domain immunoglobulin domain (Ig) and GPI membrane anchor 7A Sema L Sema7a SEMAK1 SEMAL Semaphorin 7A GPI membrane anchor Semaphorin K1 Semaphorin L Semaphorin7a SEM7A_HUMAN. |
| Product description | Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes. |
| Immunogen | KLH conjugated synthetic peptide derived from human Sema7A 351-450/666 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCow |
| Concentration | 1mg/ml |
| Alternative Names | semaphorin 7A CD 108 CD108 CD108 antigen CDw108 H Sema K1 H Sema L JMH JMH blood group antigen John Milton Hagen blood group H Sema K1 John Milton Hargen human blood group Ag MGC126692 MGC126696 Sema domain immunoglobulin domain (Ig) and GPI membrane anchor 7A Sema L Sema7a SEMAK1 SEMAL Semaphorin 7A GPI membrane anchor Semaphorin K1 Semaphorin L Semaphorin7a SEM7A_HUMAN. |
|---|---|
| Molecular Weight(MW) | 66 KDa |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Note=Detected in a punctate pattern on the cell membrane of basal and supra-basal skin keratinocytes. |
| SwissProt ID | O75326 |
|---|
Application
Fig1: Sample: Lane 1: Brain (Mouse) Lysate at 30 ug Lane 2: Lung (Mouse) Lysate at 30 ug Primary: Anti-Sema7A/CD108(ER1905-75) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 66kD Observed band size : 66kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CD108) Polyclonal Antibody, Unconjugated (ER1905-75) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Sample: Cerebrum (Mouse) Lysate at 40 ug Lung (Mouse) Lysate at 40 ug Primary: Anti-CD108 (ER1905-75) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66 kD Observed band size: 63 kD
Application
Fig4: Sample: Skin (Mouse) Lysate at 40 ug Skin(Rat) Lysate at 40 ug Primary: Anti-CD108 (ER1905-75) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66 kD Observed band size: 66 kD| Positive Control | Detected in skin keratinocytes and on endothelial cells from skin blood vessels (at protein level). Expressed in fibroblasts, keratinocytes, melanocytes, placenta, testis, ovary, spleen, brain, spinal chord, lung, heart, adrenal gland, lymph nodes, thymus, intestine and kidney. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
