Application
Fig1: Overlay histogram showing HL 60 cells stained with ER1905-76 (Green line). The cells were fixed with 90% methanol (5 min) and then permeabilized with 0.01M PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ER1905-76,1μg/1x10^6 cells) for 30 min at 22℃. The secondary antibody used was fluorescein isothiocyanate goat anti-rabbit IgG (H+L) (bs- 0295G-FITC , Brillant blue line) at 1/200 dilution for 30 min at 22℃. Isotype control antibody was rabbit IgG (polyclonal,bs-0295P,Orange line) (1μg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of 20,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.Application
Fig2: Sample:A549 Cell (Human) Lysate at 40 ug Primary: Anti-CD15(ER1905-76)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58kD Observed band size: 63kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CD15) Polyclonal Antibody, Unconjugated (ER1905-76) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig4: Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-CD15 /FITC Conjugated antibody (ER1905-76-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-FITC . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | CD15 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Fut4 SSEA-1 3-FAL Alpha (1,3) fucosyltransferase Alpha 13 fucosyltransferase FucT EC 2.4.1. ELAM 1 ligand fucosyltransferase ELAM ligand fucosyltransferase ELAM1 ligand fucosyltransferase ELFT FCT3A Fuc TIV Fucosyltransferase 4 alpha 1 3 fucosyltransferase myeloid specific Fucosyltransferase 4 FucT IV FUCTIV FUT4 Galactoside 3 L fucosyltransferase Lewis X LeX SSEA 1 SSEA1 Stage specific embryonic antigen 1 FUC-TIV. |
| Product description | May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. |
| Immunogen | KLH conjugated synthetic peptide derived from human CD15 251-295/433 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Fut4
SSEA-1
3-FAL
Alpha (1 3) fucosyltransferase Alpha 13 fucosyltransferase FucT EC 2.4.1. ELAM 1 ligand fucosyltransferase ELAM ligand fucosyltransferase ELAM1 ligand fucosyltransferase ELFT FCT3A Fuc TIV Fucosyltransferase 4 alpha 1 3 fucosyltransferase myeloid specific Fucosyltransferase 4 FucT IV FUCTIV FUT4 Galactoside 3 L fucosyltransferase Lewis X LeX SSEA 1 SSEA1 Stage specific embryonic antigen 1 FUC-TIV. |
|---|---|
| Molecular Weight(MW) | 58 KDa |
| Cellular Localization | Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. |
| SwissProt ID | Q11127 |
|---|
Application
Fig1: Overlay histogram showing HL 60 cells stained with ER1905-76 (Green line). The cells were fixed with 90% methanol (5 min) and then permeabilized with 0.01M PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ER1905-76,1μg/1x10^6 cells) for 30 min at 22℃. The secondary antibody used was fluorescein isothiocyanate goat anti-rabbit IgG (H+L) (bs- 0295G-FITC , Brillant blue line) at 1/200 dilution for 30 min at 22℃. Isotype control antibody was rabbit IgG (polyclonal,bs-0295P,Orange line) (1μg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of 20,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Application
Fig2: Sample:A549 Cell (Human) Lysate at 40 ug Primary: Anti-CD15(ER1905-76)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58kD Observed band size: 63kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CD15) Polyclonal Antibody, Unconjugated (ER1905-76) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig4: Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-CD15 /FITC Conjugated antibody (ER1905-76-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-FITC . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Highest expression in stomach and colon. It is also expressed in the lung, testis, uterus, small intestine and to a lesser extent in spleen, and ovary. Present in trace amounts in brain, thymus, heart, smooth muscle, kidney and bone marrow. Not found in liver, salivary gland and pancreas. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
