Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-CD151 (ER1905-77) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kDApplication
Fig2: Blank control(Blue): HUVEC cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-CD31 antibody(ER1905-77), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange),used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.| Product Name | CD151 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD151 antigen CD151 molecule GP27 Membrane glycoprotein SFA1 MER2 PETA3 Platelet endothelial tetraspan antigen 3 RAPH SFA 1 SFA1 Tetraspanin24 TSPAN24 CD151_HUMAN Platelet-endothelial tetraspan antigen 3 PETA-3 Tetraspanin-24 Tspan-24 CD151. |
| Product description | Essential for the proper assembly of the glomerular and tubular basement membranes in kidney. |
| Immunogen | KLH conjugated synthetic peptide derived from human CD151 101-200/253 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatChickenCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | CD151 antigen CD151 molecule GP27 Membrane glycoprotein SFA1 MER2 PETA3 Platelet endothelial tetraspan antigen 3 RAPH SFA 1 SFA1 Tetraspanin24 TSPAN24 CD151_HUMAN Platelet-endothelial tetraspan antigen 3 PETA-3 Tetraspanin-24 Tspan-24 CD151. |
|---|---|
| Molecular Weight(MW) | 28 KDa |
| Cellular Localization | Membrane; Multi-pass membrane protein. |
| SwissProt ID | P48509 |
|---|
Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-CD151 (ER1905-77) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD
Application
Fig2: Blank control(Blue): HUVEC cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-CD31 antibody(ER1905-77), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange),used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.| Positive Control | Expressed in a variety of tissues including vascular endothelium and epidermis. Expressed on erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
