Application
Fig1: Sample: HepG2 (Human) CellLysate at 40 ug MCF-7 (Human) CellLysate at 40 ug Primary: Anti-CD161/NK1.1 (ER1905-78) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 25 kD| Product Name | CD161/NK1.1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD161 antigen like family member C CD161 antigen-like family member C CD161c Killer cell lectin like receptor subfamily A member 1C Killer cell lectin like receptor subfamily B member 1C Klrb1c Ly55c Lymphocyte antigen 55c Ly59 Lymphocyte antigen 59 Natural killer cell surface protein P1 40 Natural killer cell surface protein P1-40 Ly-55c NK1.1 alloantigen NKR P1.9 NKR P1C NKRP1 40 NKRP1 NKRP1.9 Nkrp1-c NKRP1C NK-1.1 Nk-1.2 NK-RP1 NK1.1 NKR-P1 Nkrp1c NKR-P1.9 NKR-P1C KLRB1_HUMAN. |
| Product description | Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. |
| Immunogen | KLH conjugated synthetic peptide derived from human CD161/NK11 151-225/225 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CD161 antigen like family member C CD161 antigen-like family member C CD161c Killer cell lectin like receptor subfamily A member 1C Killer cell lectin like receptor subfamily B member 1C Klrb1c Ly55c Lymphocyte antigen 55c Ly59 Lymphocyte antigen 59 Natural killer cell surface protein P1 40 Natural killer cell surface protein P1-40 Ly-55c NK1.1 alloantigen NKR P1.9 NKR P1C NKRP1 40 NKRP1 NKRP1.9 Nkrp1-c NKRP1C NK-1.1 Nk-1.2 NK-RP1 NK1.1 NKR-P1 Nkrp1c NKR-P1.9 NKR-P1C KLRB1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 25 KDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q12918 |
|---|
Application
Fig1: Sample: HepG2 (Human) CellLysate at 40 ug MCF-7 (Human) CellLysate at 40 ug Primary: Anti-CD161/NK1.1 (ER1905-78) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 25 kD| Positive Control | Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
