Application
Fig1: Sample: Jurkat(Human) Cell Lysate at 30 ug MOLT-4(Human) Cell Lysate at 30 ug Primary: Anti-CD162 (ER1905-80) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 41 kDApplication
Fig2: Sample: Raji(Human) Cell Lysate at 30 ug U937(Human) Cell Lysate at 30 ug Primary: Anti-CD162 (ER1905-80) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 41 kDApplication
Fig3: Blank control: U937(blue). Primary Antibody: Rabbit Anti-CD162 antibody(ER1905-80), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min).Primary antibody (ER1905-80, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.| Product Name | CD162 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 162 CD162 CD162 antigen CLA Cutaneous lymphocyte associated associated antigen P selectin glycoprotein ligand 1 P selectin glycoprotein ligand 1 precursor P-selectin glycoprotein ligand 1 PSGL 1 PSGL-1 PSGL1 Selectin P ligand SELPL_HUMAN SELPLG. |
| Product description | A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture. |
| Immunogen | KLH conjugated synthetic peptide derived from human PSGL-1 251-350/412 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg /test: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CD 162 CD162 CD162 antigen CLA Cutaneous lymphocyte associated associated antigen P selectin glycoprotein ligand 1 P selectin glycoprotein ligand 1 precursor P-selectin glycoprotein ligand 1 PSGL 1 PSGL-1 PSGL1 Selectin P ligand SELPL_HUMAN SELPLG. |
|---|---|
| Molecular Weight(MW) | 41 KDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein. |
| SwissProt ID | Q14242 |
|---|
Application
Fig1: Sample: Jurkat(Human) Cell Lysate at 30 ug MOLT-4(Human) Cell Lysate at 30 ug Primary: Anti-CD162 (ER1905-80) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 41 kD
Application
Fig2: Sample: Raji(Human) Cell Lysate at 30 ug U937(Human) Cell Lysate at 30 ug Primary: Anti-CD162 (ER1905-80) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 41 kD
Application
Fig3: Blank control: U937(blue). Primary Antibody: Rabbit Anti-CD162 antibody(ER1905-80), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min).Primary antibody (ER1905-80, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.| Positive Control | Expressed on neutrophils, monocytes and most lymphocytes. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg /test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
