Application
Fig1: Sample: Heart(Mouse) Cell Lysate at 40 ug Primary: Anti-CD167b/DDR2 (ER1905-81) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 92 kD Observed band size: 92 kDApplication
Fig2: Blank control: U87MG. Primary Antibody (green line): Rabbit Anti-CD167b/DDR2 antibody (ER1905-81) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig3: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-DDR2 (ER1905-81) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 92 kD Observed band size: 92 kD| Product Name | CD167b/DDR2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDR 2 DDR2 DDR 2 CD167b antigen Cell migration inducing protein 20 Discoidin domain containing receptor 2 Discoidin domain receptor 2 Discoidin domain receptor family member 2v Hydroxyaryl protein kinase MIG20a Migration inducing gene 16 protein Neurotrophic tyrosine kinase Neurotrophic tyrosine kinase receptor related 3v NTRKR 3 NTRKR3v Receptor protein tyrosine kinase TKTv Receptor related 3 TKTv TYRO 10 TYRO10 Tyrosine kinase receptor related to neurotrophic TRK Tyrosine protein kinase TYRO 10 Tyrosine protein kinase TYRO10 Tyrosylprotein kinase DDR2_HUMAN Discoidin domain-containing receptor 2 CD167 antigen-like family member B Discoidin domain-containing receptor tyrosine kinase 2 Neurotrophic tyrosine kinase, receptor-related 3 Receptor protein-tyrosine kinase TKT CD167b. |
| Product description | Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell differentiation, remodeling of the extracellular matrix, cell migration and cell proliferation. Required for normal bone development. Regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. Regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. Promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing. |
| Immunogen | KLH conjugated synthetic peptide derived from human CD167b 245-350/855 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1ug/test: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | DDR 2
DDR2
DDR 2
CD167b antigen
Cell migration inducing protein 20
Discoidin domain containing receptor 2
Discoidin domain receptor 2
Discoidin domain receptor family member 2v Hydroxyaryl protein kinase
MIG20a
Migration inducing gene 16 protein
Neurotrophic tyrosine kinase
Neurotrophic tyrosine kinase receptor related 3v NTRKR 3
NTRKR3v Receptor protein tyrosine kinase TKTv Receptor related 3
TKTv TYRO 10
TYRO10
Tyrosine kinase receptor related to neurotrophic TRK
Tyrosine protein kinase TYRO 10
Tyrosine protein kinase TYRO10
Tyrosylprotein kinase
DDR2_HUMAN
Discoidin domain-containing receptor 2
CD167 antigen-like family member B
Discoidin domain-containing receptor tyrosine kinase 2
Neurotrophic tyrosine kinase receptor-related 3 Receptor protein-tyrosine kinase TKT CD167b. |
|---|---|
| Molecular Weight(MW) | 92 KDa |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. |
| SwissProt ID | Q16832 |
|---|
Application
Fig1: Sample: Heart(Mouse) Cell Lysate at 40 ug Primary: Anti-CD167b/DDR2 (ER1905-81) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 92 kD Observed band size: 92 kD
Application
Fig2: Blank control: U87MG. Primary Antibody (green line): Rabbit Anti-CD167b/DDR2 antibody (ER1905-81) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig3: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-DDR2 (ER1905-81) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 92 kD Observed band size: 92 kD| Positive Control | Detected in osteocytes, osteoblastic cells in subchondral bone, bone lining cells, tibia and cartilage. Detected at high levels in heart and lung, and at low levels in brain, placenta, liver, skeletal muscle, pancreas, and kidney. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1ug/test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
