Application
l: mouse thymocyte(2% Paraformaldehyde-fixed ) Isotype Control Antibody: Rabbit IgG; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody: Rabbit Anti-CD1A antibody (ER1905-82); Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSAApplication
Fig2: Blank control(mouse):mouse spleen cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti- CD1A antibody(ER1905-82), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.Application
Fig3: Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti-CD1d (ER1905-82) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 40 kD| Product Name | CD1d |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Antigen-presenting glycoprotein CD1d CD1.1 CD1d CD1D antigen CD1D antigen d polypeptide CD1d molecule CD1D_HUMAN Cd1d1 differentiation antigen CD1 alpha 3 HMC class I antigen like glycoprotein CD1D Ly 38 MGC34622 R3 R3G1 T cell surface glycoprotein CD1d Thymocyte antigen CD1D. |
| Product description | Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse CD1d 165-270/336 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Antigen-presenting glycoprotein CD1d CD1.1 CD1d CD1D antigen CD1D antigen d polypeptide CD1d molecule CD1D_HUMAN Cd1d1 differentiation antigen CD1 alpha 3 HMC class I antigen like glycoprotein CD1D Ly 38 MGC34622 R3 R3G1 T cell surface glycoprotein CD1d Thymocyte antigen CD1D. |
|---|---|
| Molecular Weight(MW) | 36 KDa |
| Cellular Localization | Cell membrane, Single-pass type I membrane protein. Basolateral cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Note=Subject to intracellular trafficking between the cell membrane, endosomes and lysosomes. |
| SwissProt ID | P11609 |
|---|
Application
l: mouse thymocyte(2% Paraformaldehyde-fixed ) Isotype Control Antibody: Rabbit IgG; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody: Rabbit Anti-CD1A antibody (ER1905-82); Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA
Application
Fig2: Blank control(mouse):mouse spleen cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti- CD1A antibody(ER1905-82), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Application
Fig3: Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti-CD1d (ER1905-82) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 40 kD| Positive Control | Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
