Application
Fig1: Blank control(blue):Raji cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- CD23 antibody(ER1905-85), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.Application
Fig2: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti- CD23 (ER1905-85) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 50/40 kDApplication
Fig3: Sample: 293T Cell (Human) Lysate at 30 ug Primary: Anti- CD23 (ER1905-85) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 50/40 kD| Product Name | CD23 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Fc EpsilonR II FcεRII Blast 2 Blast2 C type lectin domain family 4 member J C-type lectin domain family 4, member J CD 23 CD 23A CD23 antigen CD23A CLEC 4J CLEC4J Fc epsilon RII Fc fragment of IgE low affinity II receptor for Fc fragment of IgE, low affinity II, receptor for (CD23) Fc of IgE, low affinity II, receptor for (CD23) Fc receptor IgE low affinity II alpha polypeptide Fc receptor, IgE, low affinity II, alpha polypeptide, isoform CRA_a FCE 2 FCE2 FCER 2 FCER2 FCER2A IgE binding factor IgE receptor lymphocyte IgE-binding factor IGEBF Immunoglobulin E binding factor Immunoglobulin E receptor, low affinity II Low affinity immunoglobulin epsilon Fc receptor Low affinity immunoglobulin epsilon Fc receptor membrane bound form Low affinity immunoglobulin epsilon Fc receptor soluble form Ly-42 Ly42 Lymphocyte antigen CD23 Lymphocyte IgE receptor MGC93219. |
| Product description | Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). |
| Immunogen | KLH conjugated synthetic peptide derived from human CD23 221-321/321 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Fc EpsilonR II
FcεRII
Blast 2
Blast2
C type lectin domain family 4 member J
C-type lectin domain family 4 member J CD 23 CD 23A CD23 antigen CD23A CLEC 4J CLEC4J Fc epsilon RII Fc fragment of IgE low affinity II receptor for Fc fragment of IgE low affinity II receptor for (CD23) Fc of IgE low affinity II receptor for (CD23) Fc receptor IgE low affinity II alpha polypeptide Fc receptor IgE low affinity II alpha polypeptide isoform CRA_a FCE 2 FCE2 FCER 2 FCER2 FCER2A IgE binding factor IgE receptor lymphocyte IgE-binding factor IGEBF Immunoglobulin E binding factor Immunoglobulin E receptor low affinity II Low affinity immunoglobulin epsilon Fc receptor Low affinity immunoglobulin epsilon Fc receptor membrane bound form Low affinity immunoglobulin epsilon Fc receptor soluble form Ly-42 Ly42 Lymphocyte antigen CD23 Lymphocyte IgE receptor MGC93219. |
|---|---|
| Molecular Weight(MW) | 36 KDa |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein. Secreted. Note=Also exists as a soluble excreted form. |
| SwissProt ID | P06734 |
|---|
Application
Fig1: Blank control(blue):Raji cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- CD23 antibody(ER1905-85), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Application
Fig2: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti- CD23 (ER1905-85) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 50/40 kD
Application
Fig3: Sample: 293T Cell (Human) Lysate at 30 ug Primary: Anti- CD23 (ER1905-85) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 50/40 kD| Application Notes | WB:1:500-2000: FC:1μg/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
