Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CD30) Polyclonal Antibody, Unconjugated (ER1905-88) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Blank control(blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-CD30 antibody(ER1905-88), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.| Product Name | CD30 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 30 CD30 CD30 antigen CD30L receptor Cytokine receptor CD30 D1S166E KI 1 Ki 1 antigen Ki-1 antigen KI1 Lymphocyte activation antigen CD30 TNFRSF 8 TNFRSF8 TNFRSF8 TNR8_HUMAN Tumor Necrosis Factor Receptor Superfamily Member 8 Tumor Necrosis Factor Receptor Superfamily Member 8. |
| Product description | Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. |
| Immunogen | KLH conjugated synthetic peptide derived from human CD30 131-230/595 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:0.2μg/Test: |
|
| Species Reactivity | HumanPigRabbit |
| Concentration | 1mg/ml |
| Alternative Names | CD 30 CD30 CD30 antigen CD30L receptor Cytokine receptor CD30 D1S166E KI 1 Ki 1 antigen Ki-1 antigen KI1 Lymphocyte activation antigen CD30 TNFRSF 8 TNFRSF8 TNFRSF8 TNR8_HUMAN Tumor Necrosis Factor Receptor Superfamily Member 8 Tumor Necrosis Factor Receptor Superfamily Member 8. |
|---|---|
| Molecular Weight(MW) | 62 KDa |
| Cellular Localization | Isoform Long: Cell membrane; Single-pass type I membrane protein.;Isoform Short: Cytoplasm. |
| SwissProt ID | P28908 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CD30) Polyclonal Antibody, Unconjugated (ER1905-88) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Blank control(blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-CD30 antibody(ER1905-88), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:0.2μg/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
