Application
Fig1: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-CD38 (ER1905-91) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 48 kDApplication
Fig2: Sample: Pancreas (Mouse) Lysate at 40 ug Primary: Anti-CD38 (ER1905-91) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 48 kDApplication
Fig3: Sample: SP2/0(Mouse) Cell Lysate at 30 ug Primary: Anti-CD38 (ER1905-91) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 54 kDApplication
Fig4: Blank control: mouse spleen cells (blue). Primary Antibody: Rabbit Anti- CD38 antibody(ER1905-91), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (ER1905-91, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.| Product Name | CD38 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 2'-phospho-ADP-ribosyl cyclase 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase 2'-phospho-cyclic-ADP-ribose transferase ADP-ribosyl cyclase 1 ADPRC 1 Cyclic ADP-ribose hydrolase 1 cADPr hydrolase 1 T10 CD38_HUMAN CD38_MOUSE I-19 NIM-R5 antigen. |
| Product description | The protein encoded by this gene is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia. Alternative splicing results in multiple transcript variants. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse CD38 101-200/304 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 2'-phospho-ADP-ribosyl cyclase 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase 2'-phospho-cyclic-ADP-ribose transferase ADP-ribosyl cyclase 1 ADPRC 1 Cyclic ADP-ribose hydrolase 1 cADPr hydrolase 1 T10 CD38_HUMAN CD38_MOUSE I-19 NIM-R5 antigen. |
|---|---|
| Molecular Weight(MW) | 34 KDa |
| Cellular Localization | Membrane; Single-pass type II membrane protein. |
| SwissProt ID | P56528 |
|---|
Application
Fig1: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-CD38 (ER1905-91) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 48 kD
Application
Fig2: Sample: Pancreas (Mouse) Lysate at 40 ug Primary: Anti-CD38 (ER1905-91) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 48 kD
Application
Fig3: Sample: SP2/0(Mouse) Cell Lysate at 30 ug Primary: Anti-CD38 (ER1905-91) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 54 kD
Application
Fig4: Blank control: mouse spleen cells (blue). Primary Antibody: Rabbit Anti- CD38 antibody(ER1905-91), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (ER1905-91, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.| Positive Control | Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
