Application
Fig1: Sample: MOLT-4(Human) Cell Lysate at 30 ug Primary: Anti- CD53 (ER1905-94) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 25 kDApplication
Fig2: Sample: Molt-4(Human) Cell Lysate at 30 ug Primary: Anti- CD53 (ER1905-94) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 26 kDApplication
Fig3: Sample: Jurkat(Human) Cell Lysate at 30 ug Molt-4(Human) Cell Lysate at 30 ug Primary: Anti- CD53 (ER1905-94) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 24 kD| Product Name | CD53 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AI323659 Antigen MOX44 identified by monoclonal MRC-OX44 CD 53 CD53 CD53 antigen CD53 antigen, isoform CRA_b CD53 glycoprotein Cd53 molecule CD53 tetraspan antigen CD53_HUMAN Cell surface glycoprotein CD53 Leukocyte antigen MRC OX-44 Leukocyte surface antigen CD53 MOX 44 MOX44 OTTHUMP00000059505 Ox-44 OX44 RATOX44 Tetraspanin 25 Tetraspanin-25 Tetraspanin25 Transmembrane glycoprotein TSPAN 25 Tspan-25 TSPAN25. |
| Product description | Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human CD53 65-160/219 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | AI323659
Antigen MOX44 identified by monoclonal
MRC-OX44
CD 53
CD53
CD53 antigen
CD53 antigen isoform CRA_b CD53 glycoprotein Cd53 molecule CD53 tetraspan antigen CD53_HUMAN Cell surface glycoprotein CD53 Leukocyte antigen MRC OX-44 Leukocyte surface antigen CD53 MOX 44 MOX44 OTTHUMP00000059505 Ox-44 OX44 RATOX44 Tetraspanin 25 Tetraspanin-25 Tetraspanin25 Transmembrane glycoprotein TSPAN 25 Tspan-25 TSPAN25. |
|---|---|
| Molecular Weight(MW) | 24 KDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P19397 |
|---|
Application
Fig1: Sample: MOLT-4(Human) Cell Lysate at 30 ug Primary: Anti- CD53 (ER1905-94) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 25 kD
Application
Fig2: Sample: Molt-4(Human) Cell Lysate at 30 ug Primary: Anti- CD53 (ER1905-94) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 26 kD
Application
Fig3: Sample: Jurkat(Human) Cell Lysate at 30 ug Molt-4(Human) Cell Lysate at 30 ug Primary: Anti- CD53 (ER1905-94) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 24 kD| Positive Control | B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
