Application
Fig1: Sample: Lymph node (Mouse) Lysate at 40 ug Lymph node (Rat) Lysate at 40 ug Primary: Anti- CD62L (ER1905-95) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 43 kDApplication
Fig2: Blank control: mouse spleen cells (blue). Primary Antibody: Rabbit Anti- CD62L antibody(ER1905-95), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (ER1905-95, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.| Product Name | CD62L |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | L-Selectin A.11 AI528707 CD62 antigen ligand CD62 antigen-like family member L gp90 MEL hLHRc IgA nephropathy, susceptibility to, included L Selectin LAM1 LECAM1 LEU8 Leukocyte adhesion molecule 1 Leukocyte surface antigen Leu8 Leukocyte-endothelial cell adhesion molecule 1 LNHR LSEL Ly-22 Ly-m22 Lyam-1 LYAM1 Lymph node homing receptor Lymphocyte adhesion molecule 1 Lymphocyte antigen 22 Lymphocyte surface MEL-14 antigen PLNHR Selectin L Selectin, lymphocyte SELL TQ1 LYAM1_MOUSE. |
| Product description | This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse CD62L 301-372/372 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
|
| Species Reactivity | MouseRatCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | L-Selectin
A.11
AI528707
CD62 antigen ligand
CD62 antigen-like family member L
gp90 MEL
hLHRc
IgA nephropathy susceptibility to included L Selectin LAM1 LECAM1 LEU8 Leukocyte adhesion molecule 1 Leukocyte surface antigen Leu8 Leukocyte-endothelial cell adhesion molecule 1 LNHR LSEL Ly-22 Ly-m22 Lyam-1 LYAM1 Lymph node homing receptor Lymphocyte adhesion molecule 1 Lymphocyte antigen 22 Lymphocyte surface MEL-14 antigen PLNHR Selectin L Selectin lymphocyte SELL TQ1 LYAM1_MOUSE. |
|---|---|
| Molecular Weight(MW) | 37 KDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein. |
| SwissProt ID | P18337 |
|---|
Application
Fig1: Sample: Lymph node (Mouse) Lysate at 40 ug Lymph node (Rat) Lysate at 40 ug Primary: Anti- CD62L (ER1905-95) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 43 kD
Application
Fig2: Blank control: mouse spleen cells (blue). Primary Antibody: Rabbit Anti- CD62L antibody(ER1905-95), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (ER1905-95, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.| Positive Control | Expressed in B-cell lines and T-lymphocytes. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
