Application
Fig1: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- CD75 (ER1905-97) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 48 kDApplication
Fig2: Sample: MCF-7(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Siha(Human) Cell Lysate at 30 ug Primary: Anti- CD75 (ER1905-97) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 48 kD| Product Name | CD75 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 6 sialyltransferase 6-sialyltransferase 1 6-ST 1 Alpha 2 Alpha 2,6 ST 1 Alpha 2,6 ST B cell antigen CD75 B-cell antigen CD75 Beta galactoside alpha 2,6 sialyltransferase 1 Beta-galactoside alpha-2 CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1 CMP-N-acetylneuraminate-beta-galactosamide-alpha-2 MGC48859 Sialyltransferase 1 (beta galactoside alpha 2,6 sialyltransferase) Sialyltransferase 1 SIAT1 SIAT1_HUMAN ST6 beta galactosamide alpha 2,6 sialyltranferase 1 ST6Gal I ST6GAL1 ST6GalI ST6N. |
| Product description | Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates. |
| Immunogen | KLH conjugated synthetic peptide derived from human CD75 (321-370aa) 301-406/406 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICC/IF |
| WB:1:500-2000: IF:1:100-500: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | 6 sialyltransferase
6-sialyltransferase 1
6-ST 1
Alpha 2
Alpha 2 6 ST 1 Alpha 2 6 ST B cell antigen CD75 B-cell antigen CD75 Beta galactoside alpha 2 6 sialyltransferase 1 Beta-galactoside alpha-2 CMP N acetylneuraminate beta galactosamide alpha 2 6 sialyltransferase 1 CMP-N-acetylneuraminate-beta-galactosamide-alpha-2 MGC48859 Sialyltransferase 1 (beta galactoside alpha 2 6 sialyltransferase) Sialyltransferase 1 SIAT1 SIAT1_HUMAN ST6 beta galactosamide alpha 2 6 sialyltranferase 1 ST6Gal I ST6GAL1 ST6GalI ST6N. |
|---|---|
| Molecular Weight(MW) | 47 KDa |
| Cellular Localization | Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Secreted. Note=Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid. |
| SwissProt ID | P15907 |
|---|
Application
Fig1: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- CD75 (ER1905-97) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 48 kD
Application
Fig2: Sample: MCF-7(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Siha(Human) Cell Lysate at 30 ug Primary: Anti- CD75 (ER1905-97) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 48 kD| Application Notes | WB:1:500-2000: IF:1:100-500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
