Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Cdc14A) Polyclonal Antibody, Unconjugated (ER1906-04) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-Cdc14A (ER1906-04) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67 kD Observed band size: 67 kD| Product Name | Cdc14A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC 10 Cdc 14 Cdc 14A Cdc 14A1 Cdc 14A2 CDC10 Cdc14 CDC14 cell division cycle 14 homolog A CDC14 homolog A Cdc14A1 Cdc14A2 Cell division cycle 14 homolog A Dual specificity protein phosphatase CDC 14A Dual specificity protein phosphatase CDC14A hCDC14 CC14A_HUMAN. |
| Product description | Overproduction of hCdc14A leads to progressive cell death, accompanied by accumulation of pre-G1 DNA fragments and gradual elimination of cells at the G2/M transition. In 50% of the mitotic cells, multipolar mitotic spindles and misaggregated chromosomes are found. hCdc14A localizes to the centrosomes at the cell-cycle interphase stage. When the cell enters mitosis, this localization disappears suggesting that hCdc14A dissociates from the centrosomes at the G2/M transition. hCdc14A localizes to interphase centrosomes, but not to mitotic centrosomes, while hCdc14B localizes to the interphase nucleolus. Thus each isoform regulates separate cell cycle events. Cdc14A may affect cell cycle progression by its ability to dephosphorylate p53 at Ser315. This phosphatase activity is mediated by the interaction between the N-terminus of hCdc14A and the C-terminus of p53. |
| Immunogen | KLH conjugated synthetic peptide derived from human Cdc14A 51-150/594 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | CDC 10 Cdc 14 Cdc 14A Cdc 14A1 Cdc 14A2 CDC10 Cdc14 CDC14 cell division cycle 14 homolog A CDC14 homolog A Cdc14A1 Cdc14A2 Cell division cycle 14 homolog A Dual specificity protein phosphatase CDC 14A Dual specificity protein phosphatase CDC14A hCDC14 CC14A_HUMAN. |
|---|---|
| Molecular Weight(MW) | 67 KDa |
| Cellular Localization | Nucleus. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle. Note=Centrosomal during interphase, released into the cytoplasm at the onset of mitosis. Subsequently localizes to the midzone of the mitotic spindle. |
| SwissProt ID | Q9UNH5 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Cdc14A) Polyclonal Antibody, Unconjugated (ER1906-04) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-Cdc14A (ER1906-04) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67 kD Observed band size: 67 kD| Positive Control | Widely expressed. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
